载微球纳米羟基磷灰石/壳聚糖复合多孔支架的制备与表征

Fabrication and characterization of n-HA/CS porous scaffold containing ALG/CS microspheres

首先通过乳化法合成海藻酸钠/壳聚糖(ALG/CS)复合微球,然后将其与纳米羟基磷灰石/壳聚糖H(n-HA/CS)复合材料混合均匀,用气体发泡法制备了载微球复合组织工程支架。并用扫描电子显微镜(SEM)、傅立叶变换红外光谱仪(IR)以及转靶X射线仪(XRD)等方法对该载微球多孔支架进行分析和表征。结果表明：n-HA/CS复合材料中无机相均匀分散在连续有机基质中,复合前后两组分均未发生明显变化；制备的载微球多孔支架中孔隙分布均匀,孔间贯通性良好,孔隙率较高；而其中的微球均呈球状,直径分布在150～350μm之间；微球表面粗糙且有大量微孔,载药后将利于药物的释放；微球在整个支架中分布均匀,而且与n-HA/CS基体材料间亲和性较高。本研究将为骨或软骨缺损提供一种性能优良且具有药物缓释功能的组织工程支架。

In the paper,ALG microspheres covered with CS （ALG/CS） were firstly prepared by emulsification method. Then these microspheres were mixed with n-HA/CS composite and were fabricated into porous n-HA/ CS scaffold carrying ALG/CS micropheres by gas-foaming together with phase-separation, Their properties were characterized by the methods of scanning electronic microscope （SEM）, X-ray diffraction （XRD） and infrared spectra （IR）, The results show that n-HA are distributed uniformly into CS phase and the properties of n-HA and CS are stable in the scaffold. IR analysis indicates that some interactions between them have taken place after gas-foaming process. Pores （50-400μm） are distributed uniformly in the scaffold and have high interconnection. ALG/CS microspheres （200-350μm） in the porous scaffold are spherical and contain lots of micropores distribute on their surface,which will be benefit for the release of drug from it. The research will offer a new kind of porous scaffold with good properties for the growth of cells and tissues and for the control-release of drug which will have great potential to be used for bone or cartilage repair.