HPLC同时测定脑脉泰胶囊中三七皂苷R_1与人参皂苷Rg_1、Rb_1的含量

Determination of notoginsenoside R_1,ginsenoside Rg_1,Rb_1 in Naomaitai Capsules by HPLC

目的：建立高效液相色谱法来同时测定脑脉泰胶囊（红参、三七、当归等）中三七皂苷R1、人参皂苷Rg1、Rb1含量的方法。方法：采用高效液相色谱梯度洗脱法，色谱柱为AgiLent-ODS-3（5μm，4．6mm×250mm），流动相：乙腈。水进行梯度洗脱，流速：1．0mL/min，检测波长为203nm，柱温：35℃。结果：三七皂苷R1线性浓度范围在0．205～1．539μg，r=0．9996，平均凹收率为98．07％，RSD=0．51％（n=5），人参皂苷Rg1线性浓度范围在0．838～6．285μg，r=0．9994，平均同收率为97．66％，RSD=0．53％（n=5）；人参皂苷Rb，线性浓度范围在0．822～6．165μg，r=0．9996，平均凹收率为98．19％，RSD=0．44％（n=5）。结论：本法简单准确，重复性好，可作为脑脉泰胶囊的质量控制方法。

AIM： To develop a method of determining notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 in Naomaitai Capsules （Radix et Rhizoma Ginseng Rubra, Radix et Rhizoma Notoginseng, Radix Angelicae Sinensis, etc. ） by HPLC. METHODS： Notoginsenoside R1, ginsenoside Rg, and ginsenoside Rb1 were determined by HPLC gradient elution method, Inertsil-ODS-3 （5μm4.6 mm×250 mm） column with Acetonitrile-water as the mobile phase was used for gradient elution. The flow rate was at 1.0 mL/min and the detective wavelength was at 203 nm. The column temperature was at 35℃. RESULTS ： There was a good linear relationship at a range of 0. 205 - 1. 539μg for notoginsenoside R1 （ r = 0.999 6） , the average recovery of 98.07% , and RSD of 0.51% （n=5）, 0.838 -6.285 μg for ginsenoside Rg1 （ r = 0. 999 4 ）, the average recovery of 97.66%, and RSD of 0.53% （n =5）, 0. 822-6. 165μg for ginsenoside Rb1 （r = 0. 999 6）, the average recovery of 98.19%, andRSD of 0.44% （n = 5 ）. CONCLUSION： The method is simple, reproducible and accurate with a strong specificify, and can be used for the quality control of Naomaitai Capsules.