HPLC-ELSD测定川麦冬须根大孔树脂富集物中麦冬皂苷D、D′的含量

Quantitative determination of ophiopogonin D and ophiopogonin D′ in Fibre Ophiopogon enriched extract by macroporous resin

目的：建立HPLC-ELSD法测定川麦冬须根大孔树脂富集物中麦冬皂苷D、D′的含量。方法：以麦冬皂苷D、D′为对照品，应用HPLC-ELSD法，色谱柱：APOLLO C18柱（100A,5μ，4．6mm×250mm），流动相：乙腈-水（50：50）；流速：1．0ml／mi。；柱温：25℃；检测器：蒸发光散射检测器（ELSD），雾化温度：70℃，蒸发温度：90℃，气体流速：1．5SLM。结果：麦冬皂苷D在4．424。22．12μg范围内线性良好，相关系数r=0．9997，加样回收率为：97．6％，RSD=1．51％；麦冬皂苷D′在3．848。19．24μg范围内线性良好，相关系数r=0．9998，加样回收率：98．4％，RSD=4．80％。结论：本方法线性关系良好，重现性佳。

AIM： To establish a HPLC-ELSD method for determining ophiopogonin D and ophiopogonin D′ in Fibre Ophiopogon enriched extract by macroporous resin. METHODS： The determination was performed by HPLC-ELSD on APOLLO C18 column （100 A,5μ,4.6 mm×250 mm） ,using acetonitrile-H2O（50： 50） as a mobile phase, flow rate was at 1.0 mL/min, column temperature was at 25℃, detector： ELSD （ Neu ： 70℃, Eva ： 90℃, gas flow ： 1.5 SLM）. RESULTS ： The linear range of ophiopogonin D was 4.424-22.12μg, r = 0. 999 7, The average recovery was 97.6% ,RSD = 1.51%. The linear range of ophiopogonin D′ was 3. 848-19.24μg,r= 0. 999 8. The average recovery was 98.4% ,RSD = 4.8%. CONCLUSION： The method is convenient, sensitive and accurate.