摘要
以牛心肌为原料,采用分段盐析法提取肌红蛋白,离心透析后,用Sephadex G-100柱层析纯化。电泳结果表明,纯化后的肌红蛋白为一条粗带,与纯品肌红蛋白比较位置完全相同,试验证试采用分段盐析法提取,Sephadex G-100柱层析纯化方法可获得肌红蛋白纯品。
Myoglobin was extracted from cattle heart muscle by fractional salting out. After centrifugal and dialysis, the sample was purificed by Sephadex G-100 column chromatography. The results obtained electroresis show that there was one band. It was confirmed that sample was purified Myoglobin in comparison to purity Myoglobin.
出处
《家畜生态学报》
2006年第5期67-68,72,共3页
Journal of Domestic Animal Ecology
基金
陕西省科技创新计划项目(2004ZK03-02)资助
关键词
牛心肌
肌红蛋白
提取纯化
电泳鉴定
cattle heart muscle
myoglobin
purification
electroresis appraisal
