蓝马鸡和藏马鸡血清淀粉酶和酯酶同工酶酶谱

Isozyme-spectra of Serum Amylase and Esterase in Blue and White Eared Pheasants

采用聚丙烯酰胺凝胶垂直平板电泳法对19只蓝马鸡和15只藏马鸡血清淀粉酶和血清酯酶同工酶酶谱进行了研究。结果发现:(1)蓝马鸡和藏马鸡血清淀粉酶有AMY1,AMY2,AMY3三种同工酶,其中AMY1和AMY2存在多态性;(2)两种马鸡的血清酯酶均存在ES1,ES2和ES3三种同工酶;蓝马鸡的ES1同工酶存在显现酶活性的ES1A(36.8%)和不显现酶活性的ES10(63.2%)2种表型,而藏马鸡全部为ES10型。

19 blue-eared pheasants and 15 white-eared pheasants were used to measure the isozyme-spectra of serum amylase and esterase by means of Polyacrylamide gel electrophoresis in vertical plate. The results were as follows ： ①There were three isozymes---AMYl, AMY2 and AMY3 in serum of both eared pheasants, of thcm there was the polymorphism on AMY1 and AMY2 isozymes; ②there were three isozymes---ES1, ES2 and ES3 in serum of both eared pheasants. The ES1 isozyme presented two phenotypes---ES1 type which showed enzyme activity （36. 8% ） and ES10 type which did not show enzyme activity（63.2% ） in blue-eared pheasant while only ES10 type in white-eared pheasant.