抗鸡传染性法氏囊病病毒单克隆抗体识别的抗原表位分析

Analysis of Antigenic Epitopes Recognized by Anti-IBDV Monoclonal Antibody

用鸡传染性法氏囊病病毒(IBDV)及其重组表达蛋白VP2,VP3和单抗做各种ELISA试验,测定单抗识别的IBDV蛋白抗原及其多肽片段,并分析单抗识别IBDV抗原表位的空间关系。结果表明,抗IBDV单抗A,B,C,D,E,G与重组表达蛋白VP2呈阳性反应,而与表达蛋白VP3呈阴性反应,单抗C与表达蛋白VP2和VP3均呈阳性反应;单抗A,B,D,E,G识别IBDV VP2蛋白上相近或相似的抗原表位,均识别VP2蛋白多肽序列PJ1,PJ2,PJ5,PJ14,属于同一类群的构像性单克隆抗体。

The epitopes recognized by the 5 anti-IBDV monoclonal antibodies（Mabs） of A, B, D, E and G were defined hy ELISAs using Mahs, IBDV and its proteins expressed in Eseherichia coli and synthetic peptides. All of the5 Mabs reacted with recombinant VP2 protein hut not with recombinant VP3 protein by indirect EI.ISA, and were shown to recognize the same or closely adjacent epitopes on IBDV VP2 protein in ELISA additive test. The reactivity of each of the 5 Mahs with IBDV was clearly inhihited by the peptides of PJ1, PJ2, PJ5 and PJ14 respectively in antigen captured-ELISA. The results indicated that the 5 Mabs belong to one group of conformationaldependent Mabs which recognize the same or closely adjacent epitopes on VP2 protein of IBDV, and all recognize the peptides of PJ1, PJ2, PJ5 and PJ14.