SDS-PAGE电泳在肉苁蓉种间鉴定中的应用

Application of Soluble Proteins SDS-PAGE on the Identification of Cistanche Hoffing. et Link

目的:对肉苁蓉属4个种的种子进行鉴别。方法:可溶性蛋白质SDS-PAGE凝胶电泳。结果:肉苁蓉属种子的SDS-PAGE图谱存在显著差异,其多态性条带为81.82%。结论:各个种种子的电泳图谱可以相互区别,肉苁蓉属可溶性蛋白的SDS-PAGE图谱可作为种间鉴定的蛋白指纹图谱。

Objective： To identify 4 species of Cistanche Hoffing. et Link. Method：SDS-PAGE electrophoresis of soluble proteins. Results： Their degrees of polymorphism were 81.82%. Each species had its own unique band pattern distinguished from the others. Conclusion：The electrophoresis pattern of SDS-PAGE of soluble proteins can be regarded as protein fingerprints for identification of Cistanche Hoffing. et Link .