尿多酸肽对外周血Th1淋巴细胞分泌白细胞介素-2和干扰素-γ的影响

Effect of uroacitides injection on the function of the helper T1 lymphocytes in the secretion of interleukin-2 and interferon-γ in vitro

目的:探讨尿多酸肽对外周血辅助性T1淋巴细胞(Th1)分泌白细胞介素-2(IL-2)和干扰素-γ(IFN-γ)的影响。方法:选择肿瘤患者和健康查体者各30例,抽取静脉血,分离外周血单个核细胞(PBMCs),用10％胎牛血清1640培养液将其浓度调成1×106个·mL-1,分別加入不同浓度的尿多酸肽注射液,培养72 h后,收集细胞悬液-70℃冻存,经反复冻融、离心收集上清液,用酶联免疫吸附法(ELISA)测定上清液中IL-2和IFN-γ的含量。结果:在加入不同浓度(0,0．1,0．2．0．4 mg·mL-1)的屎多酸肽培养后,肿瘤患者PBMCs上清液中IL-2含量分别为(91．10±35．80),(40．25±14.38),(33．40±13．42)和(21．35±9．58)pg·mL-1,健康人分别为(127.76±47．91), (73.43±29．77)．(51．62±19．85)和(34．78±14．95)pg·mL-1;肿瘤患者PBMCs上清液中IFN-含量分别为(183．38±66．75),(107．48±40．33),(79.86±31．03)和(52．02±22．67)pg·mL-1,健康人分别为(249．45±89．69),(160．12±62．08),(105．04±39．47)和(69．58±26．25)pg·mL-1。在相同尿多酸肽浓度下,患者的IL-2和IFN-γ含量低于健康人,且有统计学差异(P<0．05);随培养液中尿多酸馱浓度的增加,肿瘤患者和健康人的IL-2和IFN-γ的含量逐渐降低,且有显著差异(P<0．05)。结论:肿瘤患者的Th1细胞的免疫功能低于健康人;

Objective： To analyze possible roles of variable concentrations of uroacitides for injection on the cytokines levels mediated by the helper T1 lymphocytes （Th1）. Methods ： The peripheral blood mononuelear cells （PBMCs） were extracted from the blood samples of tumor patients and healthy volunteers （30 subjects each）. The PBMCs were plated at a density of 106 cells·mL^-1 in RPMI 1640 and then mixed with uroacitides for injection of different concentrations. After incubated for 72 hours, the PBMCs were harvested and stored at -70 ℃. After repeatedly frozen and thawed, the incubated PBMCs were centrifuged and the supernatant was gathered. The concentrations of interleukin-2 （IL-2） and interferon-γ（IFN-γ） in the supernatant were assayed by ELISA. Results： After incubation with different concentration of uroactides for injection （0,0. 1,0.2,0.4 mg·mL^-1 ）, the IL-2 concentrations in the supernants of the patients＇ and healthy volunteers＇ PBMCs were （91.10 ± 35.80） vs. （ 127.76 ± 47.91）, （40.25 ±14.38）vs.（73.43±29.77）, （33.40±13.42）vs.（51.62±19.85） and （21.35 ± 9.58）vs. （34.78 ± 14.95） pg·mL^-1, respectively. The concentrations of IFN-γ were （ 183.38 ± 66.75 ） vs. （249.45 ± 89.69 ）, （ 107.48 ± 40. 33 ） vs. （ 160. 12 ± 62. 08 ）, （79.86 ± 31.03 ） vs. （ 105.04 ± 39, 47） and （52.02 ± 22.67 ） vs. （69.58 ± 26.25 ） pg·mL^-1, respectively ; the P values showed the statistical difference （P 〈 0.05 ）. The IL-2 and IFN-γ levels were inversely proportioned to the increased concentrations of uroacitides for injection （P 〈 0.05 ）. Conclusion： The tumor patients deteriorate the cell-mediated immunity function of Thl cells more than the healthy volunteers. Uroacitides inhibits the cytokine secretion mediated by Thl cells in vitro.