摘要
目的建立红细胞(RBC)和血小板(PLT)比率测定方法和血小板计数(BPC)参考方法,研究上海地区人群RBC∶PLT比率的基准值。方法采用国际血液学标准化委员会(ICSH)推荐的荧光双标记单克隆抗体流式细胞术(FCM)的参考方法,测定血液中RBC∶PLT,并与经校准的血液分析仪结果进行比较。结果上海地区正常人RBC∶PLT为21.91±4.32,与全自动血液分析仪结果之间差异无显著性(P>0.05)。结论采用FCM和荧光双标记单克隆抗体测定血液中RBC∶PLT比率是一种准确可靠的方法。
Objective To establish platelet （PLT） count and red blood cell （RBC）/PLT ratio reference method by flow cytometry. Methods The counting of specifically labeled platelets was determined by flouroytometry recommended International Committee for Standardization in Haematology （ICSH）. The result was compared with calibrated automated hematology analyzer（AHA）. Results The mean value of RBC/PLT ratio was 21.91 ±4.32. There was no difference between the reference method and AHA method （P 〉 0.05）. Conclusions This reference method for the measurement of RBC/PLT ratio is accurate and reliable.
出处
《检验医学》
CAS
北大核心
2006年第6期621-624,共4页
Laboratory Medicine
基金
上海市卫生局青年科研基金项目资助(024Y36)
