正交试验法优选羊栖菜多糖酶法提取工艺

Study on extraction process of polysaccharide from Sargassum fusiforme by enzymatic treatment

目的:优选羊栖菜多糖酶法的最佳提取工艺。方法:采用正交试验法进行优选,以褐藻胶得率和羊栖菜粗多糖含量为考察指标,对影响羊栖菜多糖酶法提取工艺的因素进行了研究。结果:酶量和酶处理温度对羊栖菜粗多糖提取有较大影响。结论:羊栖菜多糖酶法最佳提取工艺为酶处理10 m in,酶量1.2万U.100 g-1,pH 4.5,45℃。

ObJective： To investigate the optimal extraction process of polysaccharides from S.fusiforme by enzymatic treatment. Method： The optimum extraction conditions were obtained by the experiment with the orthogonal design. The content of polysaccharities of S. fus/forme was determined by spectraphotometry. Result：The amount of enzyme and temperature significantly affected total polysaccharides of S. fus/forme. Conclusion： The optimum extraction conditions include the addition of 1.2 × 10^4 U · 100 g ^-1 enzyme into water at pH 4.5, and the subsequent treatment for 10 min while the temperature is maintained at 45 ℃.