牡蛎肝宝对乙醇所致小鼠肝损伤的保护作用及其抗脂质过氧化效应

Protective effect and the anti-lipid peroxidation of taurine ganbo on hepatic injury induced by alcohol in mice

目的:了解牡蛎肝宝对乙醇所致肝病的保护作用及其抗脂质过氧化效果,为酒精性肝病的治疗提供新的途径。方法:实验于2005-08/2006-02在青岛大学医学院附属医院中心实验室完成。选择健康雌性昆明种小鼠60只,分为5组,每组12只。分为牡蛎肝宝(由中国海洋大学提供,批号为Q-02DEK001-2005,从胶州湾优质海洋生物牡蛎、扇贝、蛤蜊等提炼出的有效生物活性成分,剂量为0.4g/粒,推荐量为6粒/d)0.13,0.40,1.20g/kg(相当于人体推荐剂量的3.3,10,30倍)组,用蒸馏水稀释至所需浓度,灌胃量20mL/kg;同时设空白对照组和乙醇肝损伤模型组,乙醇肝损伤模型组用无水乙醇(分析纯),以蒸馏水稀释至50%,灌胃量12mL/kg(4800mg/kg)。空白对照组和乙醇肝损伤模型组给予蒸馏水灌胃,连续35d,1次/d。第35天各剂量牡蛎肝宝组及乙醇肝损伤模型组50%乙醇灌胃(12mL/kg)制备急性肝损伤模型,1h后各剂量牡蛎肝宝组再灌胃给予牡蛎肝宝,空白对照组和乙醇肝损伤模型组给予蒸馏水。观察各组动物体质量变化;牡蛎肝宝对肝组织中丙二醛、还原型谷胱甘肽、三酰甘油的影响;牡蛎肝宝对肝脏病理组织学变化。结果:60只小鼠均进入结果分析。①1.20g/kg牡蛎肝宝组动物实验后体质量及0.13,1.20g/kg牡蛎肝宝组的增重均低于乙醇肝损伤模型组,差异有显著性意义[分别为(27.9±2.1),(29.6±2.0)g;(9.0±2.1),(9.3±1.8),(11.2±1.9)g,P<0.05]。②1.20g/kg牡蛎肝宝组小鼠肝组织中还原型谷胱甘肽高于乙醇肝损伤模型组,差异有显著性意义[分别为(5.1±0.8),(3.7±0.5)μmol/g,P<0.05];1.20g/kg牡蛎肝宝组小鼠肝组织中的丙二醛、三酰甘油低于乙醇肝损伤模型组,差异有显著性意义[分别为(67.2±39.2),(157.2±37.1)nmol/g;(5.40±1.32),(10.20±3.58)mg/g,P<0.05]。③乙醇肝损伤模型组肝脏病理改变的程度高于空白对照组,差异有显著性意义(P<0.01);0.40,1.20g/kg牡蛎肝宝组肝脏病理改变的程度均低于乙醇肝损伤模型组,差异有显著性意义(P<0.05)。结论:牡蛎肝宝对乙醇所致的肝损伤有一定的保护作用。

AIM： To observe the protective effect and anti-lipid peroxidation of taurine ganbao on hepatic diseases induced by alcohol, to provide a new method for alcoholic hepatitis. METHODS： The experiment was performed at the Central Laboratory of Affiliated Hospital of Medical College of Qingdao University between August 2005 and February 2006. Totally 60 healthy female Kunming mice were randomly divided into 5 groups with 12 in each group： taurine ganbao （provided by China Ocean University, batch number Q-02DEK001-2005, effective bioactivity component extracted from high-quality oyster, scallop, surf clam and so on from Jiaozhou bay, the dosage of 0.4 g/granule, 6 granules a day recommendatory dosage） 0.13, 0.40 and 1.20 g/kg （3.3, 10 and 30 times of human recommendatory dosage） groups, diluted till the needed concentration by distilled water, volume of gastric perfusion of 20 mL/kg. Simultaneously, blank control group and alcohol-induced hepatic injury group （model group） were established. The model group received dehydrated alcohol （analytical pure）, and then diluted till 50% by distilled water with the volume of gastric perfusion of 12 mL/kg （4 800 mg/kg）. The blank control group and model group were treated with distilled water by gastric perfusion for 35 days, once a day. At day 35 acute hepatic injury models were established by 50% alcohol （12 mL/kg） with gastric perfusion in each taurine ganbao group and model group. One hour later, each taurine ganbao group was treated with taurine ganbao by gastric perfusion, whereas the blank control group and model group were given distilled water. Change of body mass of animals, the influences of taurine ganbao on malondialdehyde （MDA）, reduced glutathione hormone and triglyceride （TG） in hepatic tissue as well as the change of pathohistology of liver were observed. RESULTS： Totally 60 mice were involved in the result analysis.①The body mass in the 1.20 g/kg taufine ganbao group and the increased mass in the 0.13 and 1.20 g/kg taurine ganbao groups were lower than those in the model group, and the difference was signifieant [（27.9±2.1）, （29.6±2.0） g; （9.0±2.1）, （9.3±1.8）, （11.2±1.9） g,respectively, P 〈 0.05]. ② The content of reduced glutathione hormone in the 1.20 g/kg taurine ganbao group was higher than that in the model group, and the difference was significant [（5.1±0.8）, （3.7±0.5）μmol/g, respectively, P 〈 0.05]. The contents of MDA and TG in the hepatic tissue of the 1.20 g/kg taurine ganbao group were lower than those in the model group, and the difference was significant [（67.2±39.2）, （ 157.2±37.1 ） nmol/g; （5.40±1.32）, （10.20±3.58） mg/g, respectively, P 〈 0.05].③The change of pathology in hepatic tissue of the model group was larger than that in the blank control group, and the difference was significant （P 〈 0.01）. The change of pathology in the hepatic tissue of the 0.40 and 1.20 g/kg groups was smaller than that in the model group, and the difference was significant （P 〈 0.05）. CONCLUSION： The taurine ganbao has protective effect for alcoholic liver diseases.