白茅根抗炎的药理作用

Anti-inflammatory effect of imperata cylindrica

目的:观察白茅根的抗炎药理作用方法:实验于2005-11/2006-05在河南南阳医学高等专科学校和河南大学药学院药理实验室进行。选择NIH纯种健康小白鼠72只,清洁级,体质量(20±2)g;昆明种小鼠112只,清洁级,体质量(20±2)g;均由河南省南阳理工学院医学实验动物中心提供,合格证号:医动字第520002号。SD大鼠80只,体质量150～200g,由河南大学医学实验动物中心提供,合格证号:医动字第510005号。分5步进行实验,各步实验动物分别为NIH小鼠72只、SD大鼠40只、昆明种小鼠60只、SD大鼠40只、昆明种小鼠52只。将实验动物分为4组,对照组(灌胃生理盐水10mL/kg),白茅根水煎液1组(灌胃白茅根水煎液2.5g/kg),白茅根水煎液2组(灌胃白茅根水煎液5.0g/kg),抗炎药组(灌胃消炎痛或泼尼松)。灌胃给药后分别用二甲苯致小鼠廓耳肿胀,冰醋酸致小鼠腹腔毛细血管通透性增加,酵母多糖A、角叉菜胶、制霉菌素致大鼠或小鼠足趾肿胀,观察白茅根的抗炎作用。结果:本实验共纳入小鼠184只,182只进入结果分析;纳入大鼠80只,全部进入结果分析。①白茅根水煎液能减轻二甲苯所致小鼠耳廓肿胀(t=1.7960,P<0.05),大剂量效果更加明显(t=2.8589,P<0.01)。②能减轻角叉菜胶所致大鼠后足跖的肿胀(t=0.7517～2.7175,P<0.05),大剂量效果更加明显(t=3.2727～6.7311,P<0.01)。③能明显抑制冰醋酸所致小鼠腹腔毛细血管通透性的增加(t=1.5752,t=2.7615,P<0.05)。④能有效对抗酵母多糖A所致大鼠足趾肿胀(t=0.4295～2.3357,P<0.05);大剂量效果更加明显(t=0.4409～3.4208,P<0.01)。⑤对制霉菌素所致的小鼠足跖炎症模型无明显作用(P>0.05),消炎痛则能明显对抗制霉菌素所致的小鼠足跖炎症(P<0.01)。结论:白茅根具有一定的抗炎作用,其机制涉及对多种炎症介质的抑制。抗炎作用能部分解释白茅根生药的传统功效。

AIM： To study the anti-inflammatory effect of imperata cylindrical. METHODS： The experiment was conducted in the Laboratory of Pharmacology, College of Pharmacy of Henan University and Nanyang Medical College between November 2005 and May. 2006. Seventy-two pure NTH healthy clean mice with the body mass of （20±2） g and 112 Kunming clean mice with the body mass of （20±2） g were selected, which were provided by the Experimental animal Center of Nanyang Institute of Technology with the batch number of 520002. The Experimental Animal Center of Henan University with the batch number of 510005 provided 80 SD rats with the body mass of 150-200 g. There were 5 procedures in the experiment with 72 NIH mice, 40 SD rats, 60 Kunming mice, 40 SD rats and 52 Kunming mice in each procedure, respectively. All experimental animals were randomly divided into 4 groups： Control group （Intragastric administration normal saline at 10 mL/kg）, imperata cylindrical water decotion group Ⅰ （Intragastric administration of imperata cylindrical water decotion at 2.5 g/kg）, imperata cylindrical water decotion group Ⅱ （Intragastric administration of imperata cylindrical water decotion at 5.0 g/kg） and anti-imflammatory group （Intragastric administration of antinfan or dehydrocortisone）. Mice were made into models of auricular edema by dimethyl benzene and glacial acetic acid respectively after intragastric administration to increase abdominal capillary permeability, and models of paw-swelling were established with zymosan A, carrageenan and mystatin in rats or in mice to observe the anti-inflammatory of imperata cylindricas. RESULTS： Of 184 enrolled mice, 182 mice were involved in the analysis of results and all rats entered the final analysis. ① Imperata cylindrical water decotion could relieve the auricular edema in mice induced by dimethyl benzane （t=1.796 0, P 〈 0.05）, moreover, the effect at large dose was much more better （t=2.858 9, P 〈 0.01）. ② It could ameliorate the paw-swelling in rats induced by carrageenan （t=0.751 7-2.717 5, P 〈 0.05）, and the effect at large dose was much more significant （t=3.272 7 -6.731 1, /9 〈 0.01）. ③ It could significantly suppress the increased per meability of celiac blood capillary caused by glacial acetic acid （t=1.575 2, t=2.761 5, P 〈 0.05）. ④ It could remarkably resist pawswelling in rats induced by zymosan A （t=0.429 5-2.335 7,P 〈 0.05）, and the effect at large dose was much more marked （t=0.440 9-3.420 8, P 〈 0.01）. ⑤ There was no significant effect on mice models of paw inflammation induced by anticandine （P〉 0.05）, while antifani could significantly resist paw inflammation in mice induced by anticandine. CONCLUSION： Imperata cylindrica has certain anti-inflammatory effect, the mechanism of which is involved into the suppression of multiple mediators of inflammation. The anti-inflammation can partially illustrate the traditional effect of imperata cylindrical.