病理性瘢痕组织中survivin mRNA和C-myc、P27^(kip1)蛋白的表达

Detection of survivin mRNA and C-myc, P27^(kip1) proteins in pathologic scar tissue

目的探讨病理性瘢痕组织中survivin mRNA和C-myc、P27^(kip1)蛋白的表达情况。方法采用原位杂交和免疫组织化学染色技术,分别检测40例病理性瘢痕组织、20例非病理性瘢痕组织和20例正常皮肤组织中survivin mRNA和C-myc、P27^(kip1)蛋白的表达情况。结果病理性瘢痕、非病理性瘢痕和正常皮肤组织中survivin mRNA、C-myc蛋白和P27^(kip1)蛋白阳性表达率分别为67.5%、40.0%、20.0%,72.5%、45.0%、35.0%,55.0%、85.0%、90.0%;病理性瘢痕与非病理性瘢痕和正常皮肤组织比较,3指标差异均有统计学意义(P<0.05)。病理性瘢痕组织中,C-myc蛋白表达与survivin mRNA表达及P27kip1蛋白高表达均有相关性(P<0.05),survivin mRNA表达与P27^(kip1)蛋白高表达无相关性(P>0.05)。结论病理性瘢痕组织中survivin、c-myc表达上调,p27^(kip1)表达抑制,从而导致瘢痕形成。

Aim ： To study the expression of survivin gene and its relationship with expression of C-myc, P27^kip1 proteins in pathologic scar. Methods： The survivin mRNA, C-myc protein, and P27^kip1 protein in 40 cases of pathologic scar tissue,20 cases of normotrophie scar tissue, and 20 cases of normal skin tissae using in situ hybridization and immunohistochemical methods. Rosults： The positive rates of survivin mRNA, C-myc protein, and P27^kip1 protein in pathologic scar, normotrophic scar and normal skin tissue were 67.5% ,40.0% ,20.0% ;72.5% ,45.0% ,35.0% ; and 55.0% ,85.0% , 90.0%. Compared with normotrophie scar and normal skin cases, the expressions of survivin mRNA, C-myc protein and P27^kip1 protein in pathologic scar cases were significantly different （P 〈 0.05）. In pathologic scar tissue, C-myc had significant correlation with survivin mRNA and P27 kipl （ p 〈 0. 05 ） , but survivin mRNA had no significant correlation with P27 ^kip1 （P 〉 0.05）. Conclusion ： The expression of survivin and c-myc is up-regulated, and that of p27^kip1 is restrained in pathologic scar,which may contribute to the formation pathologic scar.