病理性瘢痕组织中TGF-β_1及其Ⅰ、Ⅱ型受体的表达

Expression of TGF-β_1 and its receptors of type Ⅰ and typeⅡ in pathologic scar tissue

目的检测病理性瘢痕组织中TGF-β1及其Ⅰ、Ⅱ型受体(TβRⅠ,TβRⅡ)。方法应用免疫组织化学SABC法检测60例病理性瘢痕(增生期瘢痕23例,退化期瘢痕9例;早期瘢痕疙瘩10例,晚期瘢痕疙瘩18例)、15例正常皮肤及15例非病理性瘢痕组织中TGF-β1、TβRⅠ和TβRⅡ的表达。结果病理性瘢痕组织中,TGF-β1、TβRⅠ、TβRⅡ阳性表达率均高于非病理性瘢痕与正常皮肤组织(P<0.01)。增生期瘢痕组织中TGF-β1、TβRⅠ、TβRⅡ阳性表达率均高于退化期瘢痕组织(P<0.05或0.01)。瘢痕疙瘩组织中,早期病变与晚期病变组织比较,TGF-β1、TβRⅠ、TβRⅡ阳性表达率差异均无统计学意义(P>0.05)。病理性瘢痕组织中,TβRⅠ、TβRⅡ表达呈正相关(r=0.53,P<0.01)。结论病理性瘢痕的发生与TGF-β信号转导异常活化有关。

Aim ： To explore the expression of TGF-β1, and its receptors TβR Ⅰ and TβR Ⅱ in the pathologic scar tissue. Methods： The expressions of TGF-β1, TβRⅠ , and TβR Ⅱ in 60 specimens of pathologic scar,15 cases of normal skin, and 15 cases of normotrophic scar were detected using immunohistochemical SABC method. Results： The positive rates of TGF-β1, TβR Ⅰ , and TβR Ⅱ in pathologic sears were higher than those in normotrophic scar and normal skin （ P 〈 0.01 ）. There were significant differences in the expressions of TGF-β1, TβRⅠ, and TβR Ⅱ between AHS and RHS tissue （P 〈 0.05 or 0.01 ）. In pathologic scars, there was positive correlation between the expression of TβR Ⅰ and that of TβR Ⅱ （r = 0.53, P 〈 0.01 ）. Conclusion： The high expression of TGF-β1, TβR Ⅰ, and TβR Ⅱ in pathologic scars suggests that TGF-β signaling pathway is activated in pathologic scar.