病理性瘢痕组织中黏着斑激酶、蛋白激酶B和核因子кB的表达

Expression of focal adhesion kinase, protein kinase B, and nuclear factor кB in pathologic scar tissue

目的探讨病理性瘢痕组织中整合素介导的磷脂酰肌醇-3-激酶(PI3K)信号转导途径中的关键酶黏着斑激酶(FAK)、蛋白激酶B(PKB)和核因子кB(NFкB)的表达。方法采用免疫组织化学方法检测40例病理性瘢痕、20例非病理性瘢痕和20例正常皮肤组织中FAK、磷酸化PKB(p-PKB)和NFкB的表达。结果病理性瘢痕组织中FAK、p-PKB、NFкB的阳性表达率较非病理性瘢痕和正常皮肤组织均升高(P<0.05)。病理性瘢痕组织中NFкB与p-PKB、FAK以及p-PKB与FAK表达均呈正相关(P均<0.05)。结论病理性瘢痕的形成可能与激活整合素介导的PI3K信号转导通路、促进成纤维细胞增殖有关。

Aim ： To explore the expressions of integrin signal pathway focal adhesion kinase （FAK） , protein kinase B （PKB） , and nuclear factor κB （NFκB） in pathologic scar. Methods： The expressions of FAK, phospho-protein kinase B （p-PKB） , and NFκB in 40 cases of pathologic scar,20 cases of non-pathologic scar, and 20 cases of normal skin tissue were detected using immunohistochemical method. Rosults： The expressions of FAK, p-PKB, and NFκB were significantly higher in pathologic scars group compared with the two control groups （ P 〈 0.05 ）. There was positive correlation between the expression of NFκB AND p-PKB, NFκB and FAK, and p-PκB and FAK patloglogic scar tissue （P 〈 0.05）. Conclusion： The activation of integrin signaling pathway in pathologic scars might involve in the formation of pathologic： scars through accelerating fibroblast proliferation.