铁剥夺对K562细胞凋亡及细胞周期的影响

Effect of iron-deprivation on apoptosis and cell cycle of K562 cell

目的观察铁剥夺诱导K562细胞的凋亡及对细胞周期的影响。方法实验组以不同浓度(25μmol/L、50μmol/L)的去铁胺处理K562细胞,25μmol/L去铁胺加等浓度的三氯化铁作对抗组,设空白对照组,分别收集不同时间点(16h、24h、32h、48h、72h)的细胞,用磷脂结合蛋白V/碘化丙啶(AnnexinV/PI)进行双标记,荧光显微镜观察凋亡细胞的形态变化。用流式细胞仪分析细胞凋亡率和细胞周期特征。结果K562细胞经25μmol/L、50μmol/L的去铁胺处理后发生了不同程度的凋亡,随着时间延长和剂量增加,细胞凋亡率增加(P<0.05)。细胞周期检测发现25μmol/L、50μmol/L的去铁胺处理K562细胞48h和72h后,G0/G1期细胞数较对照组升高,S期细胞数和细胞增殖指数均较对照组降低(P<0.05)。结论铁剥夺可抑制K562细胞增殖并诱导细胞凋亡,机制可能是通过剥夺细胞内铁阻止细胞进入S期。

Aim ： To observe the apoptosis of K562 cell induced by iron-deprivation and its effect on cell cycle. Methods：K562 cells treated with desferrioxamine （DFO） at different concentration （25 μmol/L and 50 μmol/L） were collected at different time point（ 16 h,24 h,32 h,48 h,72 h） and labelled with Annexin V/PI. K562 cells treated with DFO and FeCl3 were used as control. The morphological change of apoptotic cell was observed under the fluormnicroscope,and the characteristic of cell cycle and the rate of apoplosis was analyzed by flow eytometry. Results： With the, prolonged time and increased dosage, the rate of apoptosis increased （P 〈 0.05 ）. Compared with control group, the number of cells in G0/Gt phase increased while the number of cells in S phase and PI decreased （ P 〈0. 05 ）. Conclusion. Iron-deprivation might inhibit the growth of K562 cell, which may be carried nut by deleting intracellular iron and blocking the entrance of cells into S phase.