非霍奇金淋巴瘤组织中bcl-6及细胞凋亡检测

Detection of bcl-6 and apoptosis in non-Hodglin's lymphomas tissue

目的检测非霍奇金淋巴瘤组织中bcl-6的表达和肿瘤细胞凋亡情况。方法应用免疫组化SP法观察50例非霍奇金淋巴瘤(B细胞淋巴瘤30例,T细胞淋巴瘤20例)和30例淋巴结炎组织中bcl-6的表达水平,并应用TUNEL法检测细胞凋亡。结果淋巴结炎、B细胞淋巴瘤和T细胞淋巴瘤组织中bcl-6阳性表达率分别为40.0%、46.7%、35.0%,3组差异无统计学意义(P>0.05),但B细胞淋巴瘤组织中bcl-6强表达率达36.7%,高于T细胞淋巴瘤的20.0%和淋巴结炎组织的6.7%(P<0.05)。bcl-6阳性表达的非霍奇金淋巴瘤组织细胞凋亡指数低于阴性表达组(P<0.05)。结论B细胞淋巴瘤组织中bcl-6过表达可能与淋巴瘤的发生发展有关。

Aim ： To study the expression of bcl-6 and its relation with tumor cell apoptosis in non-Hodglin＇s lympho- mas （NHL） tissue. Methods：Immunohistochemiscal method was used to detect the expression of bcl-6 in 50 NHL samples and 30 lymphadenitis, TUNEL was used to detect cell apoptosis. Results： the expression rate of bcl-6 was 40.0% , 46.7%, and 35.0% in lymphadenitis, B cell lymphoma and T cell lymphoma, respectively. There was no significant difference in bcl-6 expression rate among 3 groups （ P 〉 0.05 ）. The intensive expression of bcl-6 in B cell lymphoma （36.7% ） was significantly higher than those in T cell lymphoma （20.0% ） and lymphadenitis （6.7% ） （P 〈 0.05 ）, The apoptotic index in NHL tissue with positive bcl-6 expression was lower than that with negative expression （ P 〈 0.05 ）, Conclusion： Over expression of bcl-6 might be related to the onset and process in B cell lymphomas.