氧化亚铁硫杆菌的生长条件及低pH值高铁离子驯化的研究

Study on growth conditions of thiobacillus ferrooxidans and adaptation of low pH and high ferrous concentrations

从云南某硫化矿酸性废水中分离出一株氧化亚铁硫杆菌，研究结果表明，最佳培养温度为30℃左右，最佳生长pH为2．0～2．5，最佳生长初始Fe^2＋浓度为0．15mol／L左右，在进行细菌培养时接种量取10％比较恰当。低pH值和高浓度Fe^2＋驯化后，在培养温度30℃、初始pH值1．5、初始Fe^2＋浓度25g／L条件下，氧化亚铁硫杆菌仍能保持一定的Fe^2＋氧化活性，6天Fe^2＋氧化率可达82．28％，Fe^2＋平均氧化速率达3．43g／L／d，pH值的变化是先上升后下降，最后稳定在1.81。

A strain of Thiobacillus ferrooxidans was isolated from the acidic wastewater of a sulfide minerals in Yunnan. The study on Thiobacillus ferrooxidans indicated that the best growth conditions： culture temperature is about 30℃, initial pH 2.0～2.5, initial ferrous ions concentrations about 0.15mol/L, and inocubtion amount of bacteria culture 10%. After the adaptation of low pH value and high ferrous ion concentrations ferrooxidans kept as usual appropriate oxidation vitality of ferrous ions. The oxidation rate of ferrous ions was 82.28% after 6 days and the average oxidation rate of ferrous ions was 3.43g/L/d when cultured at temperature 30℃, initial pH 1.5 and initial ferrous ions concentrations 25g/L. The pH initially went up and then decreased to 1.81 finally.