慢性阻塞性肺疾病患者单核细胞NF-κB活性的研究(英文)

Activation of nuclear factor kappaB of monocyte in patients with chronic obstructive pulmonary disease

目的探讨单核细胞NF-κB的激活在COPD发病机制中的作用。方法抽取31例急性加重期组和26例稳定期组的COPD患者外周血,分离单核细胞并培养,免疫细胞化学法测定核转录因子NF-κBp65的活性表达;同时检测外周血SOD、MDA、IL-8浓度,所有患者均测定肺功能(FEV1占预计值%、FEV1/FVC)。并以15例健康志愿者作为对照组。结果LPS刺激后,急性加重期组单核细胞NF-κBp65表达显著高于稳定期组及正常组(P<0.01),稳定期组高于正常组(P<0.01)。经LPS刺激后,各组单个核细胞NF-κBp65活性均显著增高,(P<0.05);外周血单核细胞NF-κB的激活与血清IL-8、MDA水平呈正相关,而与SOD呈负相关。结论NF-κB在COPD单核细胞表达细胞因子IL-8的信号转导调控中起重要作用。

Objective To investigate the role of NF-κB activation in the inflammatory mechanism of chronic obstructive pulmonary disease （COPD）. Methods Monocyte were collected from patients with COPD and were cultured, and stimulated with lipopolysaccharide （LPS） ; NF-κB p65 activation was measured by immunohistochemistry; SOD ,MDA and IL - 8 and lung function were determined synchronously. Results The NF-κB p65 was induced by LiDS in monocyte in all subjects, but it was most markedly done in COPD patients with exacerbateions; There was positive correlation between the NF- κB p65 activation of monocytes and levels of IL- 8 and MDA in circulation, but it was negative correlation to SOD. Conclusion NF-κB plays a vital role in regulating product of IL- 8 in monocyte in COPD.