异丙酚对油酸性急性肺损伤大鼠肺表面活性蛋白A及粘附因子-1的影响

Effects of Propofol on SP-A Content and ICAM-1 Expression in Oleic Acid-induced Acute Lung Injury Rats

目的研究静脉麻醉药异丙酚对油酸性急性肺损伤(ALI)大鼠肺表面活性蛋白A(SP-A)及粘附因子-1(ICAM-1)的影响。方法将80只大鼠随机分成正常对照组、ALI组和异丙酚低(4mg/(kg·h))、中(8mg/(kg·h))、高(16mg/(kg·h))剂量治疗组。各组大鼠均于药物治疗4h后行腹主动脉放血处死,取肺组织分别进行光、电镜病理观察。测定肺湿干比、肺水含量,WesternBlot法检测支气管肺泡灌洗液(BALF)中SP-A含量,分别以免疫组化(IHC)及流式细胞术(FCM)检测肺组织中ICAM-1的表达及含量。结果电镜显示ALI组大鼠肺泡Ⅱ型上皮细胞线粒体、粗面内质网及嗜锇性板层小体损伤,低、中、高剂量治疗组肺泡Ⅱ型上皮细胞细胞器损伤均有不同程度改善。与正常对照组比较,ALI组肺组织肺湿干比、肺水含量及ICAM-1表达升高(P<0·05),BALF中SP-A含量减少(P<0·05);静脉输注异丙酚使ALI大鼠肺组织肺干湿比、肺水含量及ICAM-1表达降低,BALF中SP-A含量增加(P<0·05)。结论4～16mg/(kg·h)异丙酚均可抑制油酸性ALI时ICAM-1过度表达,增加BALF中SP-A的含量,减轻肺组织的损伤程度,对油酸性ALI起到一定的保护作用,8mg/(kg·h)剂量效果较好。

OBJECTIVE： To investigate the effects of propofol and the expression of intercellular adhesion molecule - 1 （ICAM on the content of SP - A （surfactant- associated protein A） 1） in oleic acid - induced acute lung injury （ALI） rats. METHODS： A total of 80 rats were randomly divided into control group, ALl group, low-dose （4mg/（kg · h）, mediumdose （8mg/（kg · h） and high-dose （16mg/（kg · h） propofol treatment groups. The rats in each group were put to death at 4h after administration and the lung tissues were taken for pathological observation by light microscopy and electron microscopy. Lung W/ D ratio, water content in lungs were measured; the content of SP- A in bronchalveolar lavage fluid （BALF） were determined by Western Blot, and the expression of ICAM - 1 in lung tissue was measured by immunohistochcmistry （IHC） and flow cytometry （FCM） . RESULTS： Electron microscopically, the damages of mitochondrion, rough endoplasmic reticulum and osmiophilic multi lamellar body in type Ⅱ alveolar epithelial cells were noted in ALI group, and the damages of type-Ⅱ alveolar epithelial cells showed a different degree of amelioration in low, medium and high dose propofol treatment groups. propofol treatment groups. The expression of ICAM - 1, the lung water content and lung W/ D ratio were significantly increased in lung tissues while the content of SP - A in BALF were significantly decreased in ALl group as compared with control group（P〈0.05）. Lung W/D ratio, lung water content and ICAM 1 expression were decreased while the content of SP- A in BALF in ALI rats were increased through intravenous infusion of propofol （P 〈 0.05）. CONCLUSION： Propofol infusion at a dose of 4-16mg/ （kg · h） can inhibit ICAM - 1 overexpression in lung tissue with oleic acid- induced acute lung injury, increase SP- A content in BALF, lessen the degree of lung tissue injury, and it has protective effect on oleic acid - induced acute lung injury, esp. at a dose of 8 mg/（kg · h）.