玉米高光效基因PPDK在籼稻IR64中的整合及其与光合作用相关的特性分析

Integration of C_4-specific PPDK Gene of Maize to C_3 Rice IR64 and its Photosynthesis Characteristics Analysis

丙酮酸磷酸双激酶(PPDK)在C4植物光合作用途径中,是CO2固定的关键酶。将编码玉米丙酮酸磷酸双激酶(PPDK)基因的质粒通过基因枪和农杆菌介导转化导入籼稻IR64中,PCR-Southern的结果表明,玉米丙酮酸磷酸双激酶(PPDK)基因已经整合到IR64中。在温室条件下,分析了转基因IR64植株的剑叶全氮含量,结果表明,大部分的转基因IR64植株剑叶的全氮含量高于非转基因IR64对照,在转基因IR64植株中,最高的剑叶全氮含氮量为3.61%,比非转基因的IR64对照高1.07个百分点,剑叶全氮含量提高了42.1%。对转基因IR64植株的产量构成因素的分析结果表明,不同的转基因IR64植株之间,产量构成因素差异比较大,比如植株干重、收获指数等,有助于育种家们选择不同的育种材料用于水稻育种。

Pyruvate orthophosphate dikinase （PPDK） is a key enzyme in photosynthesis in some plants that exploit the C4 photosynthetic pathway for the fixation of CO2. The C4-specific PPDK encoding Pyruvate, orthophosphate dikinase were introduced into C3 plant, rice （Oryza Sativa L. cv. Indica IR64） mediated by biolistic and A - grobacterium transformation. The C4-PPDK gene of maize had been integrated to indica IR64 with PCR-Southem blotting. The total nitrogen of flag leaves of transgenic IR64 was analyzed with Kjeldahl method for quantitative determination of nitrogen, indicated that the total nitrogen of flag leaves of most of transgenic IR64 was higher than that of non-transgenic formants, control IR64 in the greenhouse. The maximum value of total nitrogen of flag leaves was 3.61% among transgenic IR64 plants, 1.07% higher than that of non-transgenic formants, control IR64. The total nitrogen of flag leaves of transgenic IR64 was increased 42.1%. The factors for yield of transgenic IR64 plants were analyzed, indicated there were more difference for factors of yield among transgenic IR64 plants in the greenhouse, i.e. dried plant weight, harvested index and so on. Thus helped rice breeders select different materials for breeding.