与大豆SMV3号株系抗性相关的分子标记的鉴定

Identification of Molecular Markers Linked to Resistance for SMV3 in Soybean

对大豆花叶病毒SMV抗性的遗传研究一直是大豆抗病遗传研究的热点之一。本研究以哈91R3-301×黑农41组合构建了遗传群体,其F2分离单株的SSR标记基因型基本符合1:2:1的比例,说明这个群体没有偏分离。根据F3株系的病情指数分布推测SMV3的F2成株抗性似乎由多基因控制。根据SSR分子标记的基因型和F2:3株系对SMV3抗病性表型结果连锁分析,推测Satt296是与大豆花叶病毒(SMV)3号株系抗性主基因连锁的分子标记,应用Joinmap作图软件将该标记定位在D1b连锁群上,这一结果与部分文献报道的研究结果一致。本研究获得的与抗性基因连锁的分子标记在其他的RIL群体中的验证得到了初步证实,推测定位在D1b连锁群上的抗性座位可能是控制SMV3的主基因之一,该标记可望应用于大豆抗SMV3的分子标记辅助选择。

Soybean mosaic virus （SMV） is one of the dangerous disease to cause significant yield losses in soybean production. Inheritance of resistance to SMV is quantitative and complex, it involves three to four major genes and several minor genes controlling the resistance to SMV. Until now the genetic mechanism of resistance to SMV is one of the hot point in field of soybean disease research. In this research we made a combination used Ha91R3-301 as maternal parent and Heinong41 as paternal parent, the segregation of F2 population derived from Ha91R3-301×Heinong41 was fitted to ratio 1：2：1 based on SSR genotypes, which indicates the population are well Mendelian segregation. It predicted that SMV race 3 resistance to F2 individuals should be controlled by multi-loci based on the analysis of the distribution of SMV 3 disease index bioassayed in F3 lines. And also association analysis between SSR genotypes and SMV resisitant phenotypes illustrated that the marker Satt296 was very close linked to the major locus conferring resistance to SMV3, which has been integrated on the linkage group D1b. This result was consistent to some previous published papers. Further studies were confirmed that the marker Satt296 could be validated in our RIL populations, which indicated that this marker would be used as marker in marker assisted selection programs