白细胞介素-1基因族多态性与脑梗死的关系

The relationship between interleukin-1 gene polymorphisms and cerebral infarction

目的探讨白细胞介素(IL)-1α及IL-1β基因多态性与脑梗死的关系。方法用聚合酶链反应-限制性片段长度多态性技术对110例脑梗死患者(梗死组)和110例健康者(对照组)的IL-1α-889C/T、IL-1β+3953C/T及IL-1β-511C/T的基因多态性进行分析。结果梗死组IL-1α-889C/T和IL-1β+3953C/T基因型的分布频率与对照组比较差异均有显著性(P=0.035,P=0.023);梗死组IL-1α-889 T、IL-1β+3953 T等位基因频率分别为13.2%和5.9%,与对照组的5.9%、1.8%比较差异有显著性(P=0.009,P=0.026);IL-1β-511C/T的基因型频率及等位基因频率两组比较差异无显著性(P=0.891,P=0.634)。结论脑梗死患者IL-1α-889T及IL-1β+3953 T等位基因频率增高,IL-1α-889C/T、IL-1β+3953C/T基因多态性与脑梗死的发病有关。

Objective To discuss the relationship between interleukin-1α （ IL-1α ）, interleukin-1β （ 1L-1β） gene polymorphisms and cerebral infarction. Methods IL-1α - 889C/T, IL-1β ＋ 3953C/T, IL-1β- 511C/T gene polymorphisms in 110 patients with cerebral infarction （cerebral infarction group） and 110 healthy individuals （control group） were analyzed with polymerase chain reaction restriction and fragment length polymorphism （PCR-RFLP）. Results There were significant differences in the distribution frequencies of IL-1α -889C/T and IL-1β ＋ 3953C/T genotypic genes in the cerebral infarction group as compared with the control group （ P = 0. 035, P = 0. 023 ） ; the allele frequencies of IL-1α - 889 T and IL-1β ＋3953 T in the cerebral infarction group were 13.2% and 5.9%, respectively, and there were significant differences as compared with 5.9% and 1.8% in the control group （P = 0. 009, P = 0. 026）. There was no significant difference between the genotype distribution frequencies of IL-1β -511C/T and the allele frequencies in both groups （P = 0. 891, P = 0. 634）. Conclusions IL-1α -889C/T and IL- 1β ＋ 3953C/T gene polymorphisms are associated with cerebral infarction and the probability of IL-1β -511C/T and IL-1β ＋ 3953 T allelic genes may increase in patients with cerebral infarction.