转SOD基因对烟草抗旱性和相关生理指标的影响

Effect of transferred SOD gene on drought resistance and related physiological parameters in tobacco

以近等基因系烟草(非转基因品系、转Fe-SOD基因品系和转Mn-SOD基因品系)为材料,研究了盆栽条件下转SOD基因对烟草抗旱性的影响。结果显示:外源Mn-SOD基因的导入能切实提高烟草抗旱能力,而导入的Fe-SOD基因虽能提高烟草体内的SOD活性水平,但不能提高烟草的抗旱性,说明Mn-SOD可能与烟草的抗旱性关系较大;当遭受干旱胁迫时,所导入的2种SOD基因可能在某种程度上影响了植物体内MDA、蛋白质、光合作用以及脯氨酸等的正常生理代谢;脯氨酸、MDA、蛋白质等生理指标的变化在抗旱与不抗旱品系之间并没有表现出明显的规律性,因此能否作为抗旱育种的重要指标应该通过更多的试验来确定。

This study aimed at revealing the effect of transferring SOD gene on drought resistance of pot-cultured tobacco near-isogenic lines （i. e. non-transgenic line, Mn-SOD gene transgenic line and Fe-SOD gene transgenic line）. The results showed that the transferred Mn-SOD gene in tobacco could actually improve the drought resistance of this crop. On the contrary, the transferred Fe-SOD gene could evidently increase SOD activity in tobacco leaves,but did not improve its drought resistance. So we deduce that Mn-SOD may have clos- er relation to drought resistance of tobacco. The two transferred SOD genes possibly affected the normal physiological metabolism of some physiological and biochemical matters such as malondiadehyde, protein,photosynthesis and proline under water stress. The changes of physiological parameters such as proline,MDA and protein did not show distinct relations with drought resistance of the tested tobacco lines, more experiments are needed to identify the importance of these parameters in selection of drought tolerant tobacco cultivars and assessment of drought resistance of tobacco.