c-Src、基质金属蛋白酶-7及金属蛋白酶组织抑制物-2在胃癌中的表达与侵袭转移的关系研究

EXPRESSION OF c-Src,MMP-7 AND TIMP-2 IN GASTRIC CANCER:EXPLORATION MECHANISM OF INVASION AND METASTASIS

目的通过对胃癌组织中c-Src、基质金属蛋白酶-7(matrix metalloproteinase-7,MMP-7)及金属蛋白酶组织抑制物-2(tissue inhibitors of meralloproteinase-2,TIMP-2)表达情况的观察,探讨它们在胃癌侵袭和转移过程中的可能作用和在预测胃癌转移潜能中的意义,为更准确的确定临床分期、判断预后、指导治疗提供依据。方法采用免疫组织化学检测50例胃癌组织及10例正常胃组织的c-Src、MMP-7及TIMP-2的表达情况。结果c-Src在胃癌中的表达与临床分期、组织学分级及淋巴结是否转移有关;MMP-7在胃癌中的表达与胃癌患者的临床分期、组织学分级及有无淋巴结转移有关;TIMP-2在胃癌组织中的表达仅与胃癌患者的临床分期有关。MMP-7蛋白表达与TIMP-2表达无明显相关。结论MMP-7、TIMP-2、c-Src同时发生异常可作为评估胃癌恶性程度的重要生物学指标。

Objective The purpose of this study was to investigate the expression of c-Src, MMP-7（matrix metalloproteinase-7） and TIMP-2 （tissue inhibitors of meralloproteinase-2） in 50 gastric carcinoma samples with lymph node metastasis and without lymph node metastasis. In order to explore the role of c-Src, MMP-7 and TIMP-2 in the metastasis and invasion of gastric carcinoma, provide the basis for confirming accurate clinical phasing, diagnositic judgment and proper treatment. Methods Fifty gastric carcinoma samples were analyzed and meanwhile 10 normal gastric mucosa samples were used as controls. S-P methods were used to detect the expression of c-Src, MMP-7 and TIMP-2 in gastric carcinoma. Results There were significant correlation among c-Src expression and clinical stages, histological grade ,lymph node metastasis; There were significant correlation among MMP-7 expression and clinical stages, histological grade, lymph node metastasis; Significant relationship was only observed between TIMP-2 expression and clinical stages. In the gastric cancer cases, No correlation found in expression level of MMP-7 and TIMP-2 was found. Conclusion To measure the expression of c-Src, MMP-7 and TIMP-2 in the same time may have a clinical significance in understanding malignant behavior of gastric cancer.