改良Potts手术建立犬单侧动力性肺动脉高压模型

Surgically Induced Unilateral Dynamic Pulmonary Hypertension Model by Modified Potts Operation in Dogs

目的建立犬单侧动力性肺动脉高压模型。方法采用改良Potts手术方法,Gore-Tex管道连接犬降主动脉与左肺动脉。于8周和12周后测定左右肺动脉压等血液动力学参数;心脏彩超或心血管造影观察分流通畅情况;并行肺组织病理学检查,评价周围肺肌性动脉肌化情况和肺肌性动脉中膜肥厚程度。结果手术分流的12只动物存活率为75%,8只动物分流管道保持通畅。8周和12周后左肺动脉压明显高于右肺及对照组(P<0.01),左、右肺动脉病理改变有明显差异(P<0.05)。光镜下观察到手术分流动物左肺肌性动脉中膜肥厚,无新生内膜形成。结论利用改良Potts手术可成功建立犬单侧动力性肺动脉高压模型。

Objective To evaluate an animal model of surgically induced unilateral dynamic pulmonary hypertension Methods In 12 mongrel dogs, the bypass was established between the left pulmonary artery and the descending thoracic aorta by Gore-Tex conduit through a left thoracotomy. In this way, increased pulmonary blood flow and systemic perfusion pressure in the left lung were obtained. After an interval of 8 to 12 weeks, the animals were performed cardiac catheterization and then were sacrificed. Histological examination was done on both lungs. Results The mean left pulmonary arterial pressure increased significantly after operation（ P 〈 0. 01 ）. Histological examination showed medial hypertrophy in all the left lungs, but neointimal proliferation was not observed. Conclusion The models of unilateral dynamic pulmonary hypertension can be induced surgically by modified Potts operation in dogs.