βB2晶体蛋白基因敲除小鼠模型的建立

Establishment of a βB2 crystallin gene knockout mice model

目的:建立βB2晶体蛋白基因敲除小鼠模型。方法:采用美国iGTL实验室的技术构建打靶载体,建立基因敲除小鼠模型。采用PCR对小鼠基因型进行鉴定,Western印迹法对晶体蛋白的表达进行鉴定。结果:PCR成功检测出3种基因型;纯合子基因敲除的小鼠未检测到βB2晶体蛋白的表达。结论:成功构建了βB2晶体蛋白基因敲除的小鼠模型。

Objective：To establish a βB2 crystallin gene knockout mice model. Methods.- The gene-targeting vector was established by the technique of American ingenious Targeting Laboratory. PCR technique was used to identify the genotype of the model mice and expression of βB2 crystallin protein was detected by Western blot. Results： Three genotypes were successfully identified and expression of βB2 crystallin protein was not detected in gene knockout mice. Conclusion： The βB2 crystallin gene knockout mice model has been successfully established.