当归对大鼠缺血性脑损伤后Flt-1、Flk-1mRNA表达的影响

EFFECTS OF ANGELICA SINESIS TREATMENT ON THE EXPRESSION OF FIT-1 AND FIK-1 mRNA AFTER THE ISCEMIC BRAIN INJURY IN RATS

目的:研究大鼠缺血性脑损伤后不同时间点Flt-1、Flk-1mRNA的表达及当归对其表达的影响。方法:雄性Wistar大鼠,随机分为缺血损伤组和当归治疗组。采用线栓法制作大鼠短暂性大脑中动脉阻断(MCAO)与再灌模型,治疗组腹腔注射当归注射液(剂量5g/kg)。36只大鼠(每组各18只)在脑缺血/再灌后1d、3d、7d神经行为学评分完成后被处死,取大脑行氯化三苯四唑(TTC)染色以测脑梗死比;另取72只大鼠(每组各36只)在脑缺血/再灌后3h、6h、12h、1d、3d、7d分别被处死,应用半定量逆转录聚合酶链反应(RT-PCR)技术检测缺血侧Flt-1、Flk-1mRNA的表达。结果:在同时间点神经功能缺损评分比较,缺血损伤组明显高于当归治疗组(P<0.05);在同时间点当归治疗组梗塞比明显小于缺血损伤组(P<0.01)。RT-PCR检测表明,缺血损伤组Flt-1、Flk-1mRNA在缺血/再灌后3h即开始表达增强,于3d达高峰,后逐渐降低;当归治疗组Flt-1、Flk-1mRNA表达比缺血损伤组明显增加,于3d达到高峰后缓慢降低至第7d仍保持较高水平。缺血损伤组和当归治疗组中Flt-1mRNA与Flk-1mRNA的表达呈正相关性,相关系数为r=0.957(P<0.01)。结论:当归可增强缺血性脑损伤后Flt-1、Flk-1mRNA表达。Flt-1、Flk-1mRNA的表达紧密相关。

Aim： To investigate the effects of Angelica sinensis on the expression of Flt-1 ,Flk-1 mRNA after the ischemic brain injury in rats. Methods： Wistar rats randomly divided into two groups： group A rats underwent middle cerebral artery oeclusion（MCAO） for 2 hours by suture, group B rats underwent MCAO for 2 hours meanwhile received treatment with Angelica sinensis（5g/kg）. At 1 st d, 3 rd d and 7 th d after reperfusion, 36 rats（ n = 18 in each group） were assessed by neurological scale and brain tissue was taken to assess the lesion ration with 2, 3, 5-triphenyltetrazolium chloride（TTC） staining. The other rats（ n = 3 at different time points in each group） were decapitated at 3 h, 6 h, 12 h , 1 st d, 3 rd d, 7 th d after reperfusion . Quantitative reverse transcription and polymerase chain reaction（RT-PCR） technique was used to examine the gene expression of Flt-1,Flk-1. Results： The neurologic deficit score of rats in group B decreased significantly compared with group A at the same time point （ P 〈 0.05）. The infarct volume of group A was significant greater than group B at the same time point after reperfusion（P〈 0.01）. The results of RT-PCR revealed that the gene expression of Flt-1 ,Flk-1 in the two groups increased from 3 h after reperfusion and reached its peak at the time of 3 rd d after reperfusion, then declined gradually. The gene expression of Flt-1 ,Flk-1 in the group B was significantly increased than group A at the same time point（P〈0.01）. The gene expression of Flk-1 was positive correlated with Flt-1 in two groups（ r = 0. 957）. Conclusion： The increasing amount of Fit-1. Flk-1 expression was enhanced by Angelica sinensis following transient interruption of cerebral blood flow in rats.