家兔发热过程中单核细胞HSF1聚合与IL-1β、TNF-αmRNA表达的关系

THE RELATIONSHIP BETWEEN THE POLYMERIZATION OF HSF1 AND THE EXPRESSION OF IL-1β、TNF-α mRNA OF MONOCYTES IN FEVER RABBITS

目的:探讨热休克因子1(HSF1)参与体温调控的作用及其生物学机制。方法:在复制家兔LPS发热模型基础上,检测在发热过程中单核细胞HSF1的表达与IL-1β、TNF-αmRNA表达之间的关系。结果:注射LPS0.5μg/kg后家兔体温明显升高,在60min和180min时出现两个体温高峰;由LPS引起发热过程中单核细胞TNF-α、IL-1βmRNA表达量分别在80min和160min达高峰,400min以内降至基础水平;单核细胞HSF1三聚体含量在体温上升到一定水平,即从注射LPS后160min开始逐渐增多。LPS致发热时单核细胞HSF1三聚体含量与单核细胞IL-1β、TNF-αmRNA表达量之间呈现负相关关系;而体温与单核细胞IL-1βmRNA表达量呈现正相关动态变化。结论:在LPS致发热时HSF1可能通过抑制IL-1β、TNF-α等内生性致热原基因的表达而限制体温升高。

For further investigating of heat shock transcription factor 1 （HSF1） action in thermoregulation and its physiological mechanism. Methyls： The relationship among the expression of HSF1 and interleukin 1β（IL-1β） mRNA and tumor necrosis factor α （TNF-α） mRNA in nomocytes was studied during the different fever stages in rabbit fever model induced by LPS. The expressions of IL-1β and TNF-α mRNA were measured by RT-PCR assay; HSF1 expression was measured by Western blot. Results： ① Intravenous injection of LPS produced a double-peak temperature arisen at 60 min and 180 min. ② The expression of IL-1β mRNA in monocytes had a peak at 160 min, while the peak of TNF-α mRNA expression occurred at 80 min after intravenous injection of LPS. ③ The content of the HSF1 trimer increased gradually after 160 min intravenous injection of LPS. The results indicated that the content of the HSF1 trimer was negative correlation with the expressions of IL-1β mRNA and TNF-α mRNA, and the change in body temperature was a positive correlation with IL-1β mRNA. Conclusion： It is possible that HSF1 limits the rise in body temperature by repressing the gene expressions of the endogenous pyrogen, IL-1β and TNF-1α.