简易快速的大鼠动脉血栓形成模型

Simple and rapid rat model of arterial thrombosis induced by ferric chloride

本研究旨在建立一个简单、快速、低廉、实用的小动物动脉血栓模型，为溶栓药物研究提供手段。经鼠右髂动脉插管至腹主动脉最下端监测血压，左颈静脉插管以备给药，用不同浓度的三氯化铁溶注浸渍过的滤纸条覆盖在分离的腹主动脉表面诱导血栓形成。结果显示：（１）１０％～７０％三氯化铁溶液能在５７±３ｍｉｎ到１４±３ｍｉｎ内形成血栓，３５％三氯化铁为最适宜的浓度；（２）３５％三氯化铁溶液诱导血栓形成时，在２６分钟后腹主动脉压下降至最低值，为血栓形成前的２３％；（３）血栓组成包括血小板、红细胞和纤维蛋白的网状结构。结果表明：三氯化铁可诱导血栓形成，符合动脉血栓特征；腹主动脉压的连续监测是判断血栓形成和溶解的简单易行的敏感指标。

The purpose of these studies were to produce a simple rapid small animal model of arterial thrombosis for study of novel antithrombotic agents. A guide was inserted into the lowest end of abdominoaorta of the anesthetized rat and abdominoaorta pressure (AAP) was recorded continuously. Arterial injury was induced by FeCl3 solution applied to pically to abdominoaorta. The results showed : (1) AAP decreased rapidly to the lowest value 23 percent (compared with control) after 26min when arterial injury was induced by 35 percent FeCl3 solution applied topically to the artery. And after thromolysis,AAP was recovered to 76 percent (compared with control ). (2) Average time to occlusion,signaled by the lowest value of AAP,ranged from 57± 3min to 14± 3min after 10 to 70 percent FeCl3 application respectively.(3)Vessel segments were fixed at various times after FeCl3 exposure and examined by scanning electron microscopy.Endothelial damage was observed and was associated with thrombus composed of activated platelet,fibrin strands and entrapped erythrocytes. The results demonstrate the FeCl3 may induced formation of an occlusive mixed thrombus,and the effect of thrombolysis was good. AAP was a simple and sensitive index of determing thrombosis.