C－sis、C－myc癌基因反义寡脱氧核苷酸抑制动脉平滑肌细胞增殖

Antisense oligodeoxynucleotides of C-sis、C-myc oncogene inhibit proliferation of arterial smooth muscle cells

用合成Ｃ－ｓｉｓ、Ｃ－ｍｙｃ癌基因反义寡脱氧核苷酸（ＡＯＤＮ）与兔动脉平滑肌细胞（ＡＳＭＣｓ）共同培养，旨在通过ＡＯＤＮ对癌基因的封闭，动态观察ＡＯＤＮ对ＡＳＭＣｓ增殖的影响，探讨ＡＯＤＮ对动脉粥样硬化（ＡＳ）尤其是冠脉成形术后再狭窄（ＲＡＣＡ）的防治作用。结果表明：①Ｃ－ｓｉｓＡＯＤＮ、Ｃ－ｍｙｃＡＯＤＮ具有明显抑制ＡＳＭＣｓ增殖作用，其抑制作用随浓度增加而加强；②Ｃ－ｓｉｓＡＯＤＮ＋Ｃ－ｍｙｃＡＯＤＮ具有协同抑制ＡＳＭＣｓ增殖作用；③Ｃ－ｓｉｓＡＯＤＮ、Ｃ－ｍｙｃＡＯＤＮ明显抑制ＤＮＡ合成，二者联合应用，具有加强抑制ＤＮＡ合成作用。研究结果提示：Ｃ－ｓｉｓ、Ｃ－ｍｙｃ癌基因ＡＯＤＮ抑制ＡＳＭＣｓ增殖，尤其是二者联合应用，有可能为ＡＳ，特别是ＲＡＣＡ防治提供一个新的途径。

To prevent the proliferation of arterial smooth muscle cells (ASMCs) in atherosclerosis (AS),especially in restenosis after coronary angioplasty (RACA), we established ASMCs model in vitro.ASMCs of rabbit were cultured with synthesized C-sis.C-myc antisense oligodeoxynucleotides (AODN) in different concentration or different time respectively,and then to assessed the inhibitory effect of AODN continuously. The result revealed : (1) C-sis AODN.C-myc AODN obviously inhibited ASMCs proliferation, the inhibitory effect was enhanced with increased concentration of AODN: (2) C-sis AODV and C-myc AODN had synergistic Inhibitory effect in ASMCs proliferation; (3) C-sis AODN .C-myc AODN obviously inhibited 3H-TdR incorporation into DNA,the inhibitory effect was intensified when C-sis AODN and C-myc AODN were used together. Our conclusion t AODN of C-sis.C-myc oncogene inhibited ASMCs proliferation,C-sis AODN and C-myc AODN had synergism in antiproliferation of ASMCs. It's probable approach to prevent AS,especially RACA, by AODN reducing ASMCs proliferation.