癫痫持续状态后海马神经元凋亡的形态学证据及意义

Evidence of Neuron Apoptoticus Morphology in Hippocampus after status Epileptic Rat

癫痫持续状态（ｓｔａｔｕｓｅｐｉｅｐｔｉｃｕｓ，ＳＥ）可造成选择性神经元损害。近来研究发现这些脑组织中神经元死亡可能是通过凋亡（ａｐｏｐｔｏｓｉｓ）机制。腹腔注射贝美格（Ｂｅｍｅｇｒｉｄｅ）诱导ＳＥ，研究了鼠ＳＥ后脑损害中的神经元凋亡现象，以ＨＥ染色、电镜、荧光（ＡｃｒｉｄｉｎｅＯｒａｎｇｅ，ＡＯ）细胞核染色方法观察细胞形态，以流式细胞术及凝胶电冰鉴定ＤＮＡ片断。ＳＥ后２４小时，海马中的易损神经元死亡具有凋亡特征：①光镜及电镜观察到细胞核内染色体凝聚和边及；②阳性ＡＯ着色细胞核；③流式细胞术及凝胶电冰显示具有ＤＮＡ片断。而且有Ⅰ型和Ⅱ型两种形式的凋亡。因此ＳＥ引起海马损害中神经元有通过调亡机制发生死亡．

Selective meuronal vulnerability is a feature of status epilepticus (SE). Recent studies have shown that certain types of nerve cell death in brain occar by a apoptotic mechanism. We have examined the role apoptosis plays in (SE ) brain damage using intraperitoneally (IP ) injection of Bemegride. With HE staining technique, electron microscop,fluorescent nuclear staining and flow cytometry, DNA ladder in agarose gel electroph,the apoptosis neurons were observed, 24 hours afaer SE, in the vulnerable neurons of the hippocampal. Including:①nuclear chromalin condensation and marginalization with light and electron microscopy; ②positive nuclear fluorescent staining (AO) ;③DNA fragmentation with a flow cytometry and ladder pattern on agarose gel electrophoresis. Therefore apoptosis contributes to the hippocumpal cell damage induce by SE.