亚低温对犬心脏骤停后脑水肿及血脑屏障的影响

The effects of mild hypothermia on brain edema and the blood-brain barrier after cardiac arrest

目的观察亚低温干预对犬心脏骤停后脑水肿及血脑屏障的影响。方法共选取16只成年健康杂种犬,将其随机分为亚低温组(n=8)和对照组(n=8),采用诱发室颤的方法导致上述2组动物心跳、呼吸骤停,随后施行脑复苏程序;亚低温组动物在心跳骤停期间给予亚低温干预。采用双抗夹心酶联免疫吸附技术测定各组动物血清S100B蛋白含量,同时观察其脑组织含水量及病理学改变。结果亚低温组动物经亚低温干预后,发现其血清S100B蛋白含量显著低于对照组(P<0．05),脑组织含水量也显著低于对照组(P <0．05);2组实验动物脑标本经病理学检测后发现,亚低温组动物脑组织缺氧损伤程度明显轻于对照组。结论亚低温干预能减轻心跳骤停实验犬的脑水肿程度,改善其血脑屏障功能,从而发挥脑保护效应。

Objective To study the effects of mild hypothermia （MH） on brain edema （BE） and blood-brain barrier （BBB） after cardiac arrest in dogs. Methods Sixteen adult mongrel dogs were randomly divided into 2 groups： a MH group （n = 8） and a control group（n = 8）. Following cardiac arrest induced by ventricular fibrillation, all the animals were subject to resuscitation, and then those in the MH group were intervened by MH. Serum S100B protein concentrations were detected using an ELISA method. The amount of water in brain tissues was measured, and any pathomorphological changes in the brain tissues were observed. Results Serum levels of S100B protein and the amount of water in brain tissues were significantly decreased in the treated animals. Pathomorphological changes to the brain tissue were also judged to be moderated in the MH group. Conclusion MH has cerebral protective effects after cardiac arrest in dogs, decreasing brain edema and preserving the blood-brain barrier.