非霍奇金淋巴瘤患者红细胞CR_1密度基因多态性及其与淋巴细胞和红细胞免疫功能相关性的研究

The relationship between CR_1 genomic density polymorphism on erythrocyte and immune function of lymphocytes and erythrocytes of patients with non Hodgkin lymphoma

日的：探讨非霍奇金淋巴瘤(non Hodgkin lymphoma,NHL)(CR_1密度基因多态性及与淋巴细胞和红细胞免疫功能的相关性。方法：96例初治的NHL患者及60例正常献血者,采用PCR加HindⅢ酶切方法测定CR_1密度基因多态性分布；采用ELISA法测定患者血清中CD4和CD8分子的含量；采用郭峰法测定患者血液中RBC-C3bRR、RBC-ICR和TRR水平。结果：(1)NHL患者红细胞CR_1密度基因组HH型比率明显低于正常人群,而HL型及LL型比率都明显高于正常人群(P＜0．01)；(2)NHL患者红细胞CR_1密度基因HH型组RBC-C3bRR、TRR、CD4、CD4/CD8比值水平明显高于HL型组(P＜0．05,P＜0．01,P＜0．05,P＜0．01),而低于正常对照组HH型组分别为(P＜0．01,P＜0．01,P＜0．05,P＜0．01),并且LL型组低于HL型组分别(P＜0．05,P＜0．01,P＜0．01,P＜0．01)；(3)NHL患者HH型组RBC-ICR明显低于HL型组(P＜0．05)而高于正常对照组HH型组(P＜0．05),并且LL型组高于HL型组(P＜0．01)；(4)NHL患者红细胞CR_1密度基因分布与红细胞及淋巴细胞免疫功能成正相关(P＜0．01)。结论：NHL患者红细胞CR_1密度基因组HH型比率明显低于正常人群,而HL型及LL型比率都明显高于正常人群(P＜0．01)；CR_1密度基因多态性与机体的红细胞及淋巴细胞免疫功能相关。

Objective： To study the relationship between the genomic density polymorphism of erythrocyte complement receptor 1 （CR1） and immune function of lymphocytes and erythrocytes of the patients with non Hodgkin lymphoma. Methods： Ninety-six patients with non Hodgkin lymphoma who received initial treatment and 60 normal volunteers were involved in this study. Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） and Hind m restriction enzyme digestion method were used to determine the distribution of CR1 genomic density polymorphism. The serum level of CD4 and CD8 was detected by enzyme-linked immunosorbant assay （ELISA）. The red blood cell-C3b receptor rosette rate （RBC-C3bRR）, RBC- immunocomplex rossete rate （RBC-ICR）, and RBC- transfusion reaction rate （TRR） were detected by the method established by Guo Feng. Results： （1）The frequency of homozygotes with CR1 high density allele （H H） was significantly lower and the frequency of homozygotes with low density allele （LL） and heterozygotes （HL） in NHL patients was significantly higher than normal control （P〈0.01）. （2）The level of RBC-C3bRR,RBC-TRR,CD4, and the ratio of CD4/CD8 in NHL patients with HH genotype were significantly lower than that of normal control with H H genotype （P〈0.01, P〈0.01, P〈0.05, P〈0.01 ）and were markedly higher than that of the NHL patients with HL genotype or LL genotype （P〈0.05, P〈0.01, P〈0.05, P〈0.01）. The level of RBC-C3bRR,RBC-TRR,CD4, and the ratio of CD4/CD8 in N HL patients with LL genotype were markedly lower than those with HL genotype （P〈0.05, P〈0.01, P〈0.01, P〈0.01）. （3）The level of RBC-ICR in NHL patients with HH genotype was significantly lower than those with HL genotype but significantly higher than normal control with HH genotype （P 〈0.05）. The level of RBC-ICR in NHL patients with LL genotype was significantly higher than those with HL genotype （P〈 0.01）. （4）There was a positive correlation between CR1 genomic densitypolymorphism and the immune function of lymphocytes or erythrocytes （P〈0.01）. Conclusion：The frequency of H H genotype was significantly lower and the frequency of LL or HL genotype was significantly higher in N HL patients than normal control （P〈0.01）. There was a positive correlation between CR1 genomic density polymorphism on erythrocyte and immune function of lymphocytes or erythrocytes in the body.