“流体力学转染”——安全高效的动物活体器官基因转染方法

Hydrodynamics-Based Transfection:A Simple,Convenient and Efficient in vivo Transgene Method

作为一种新的简便而高效的动物活体基因转染方法,“流体力学转染”技术正日益成为生物遗传学家们进行基因研究的重要工具。该技术利用“流体力学”原理,通过裸质粒溶液的静脉注射,实现了活体肝靶向性的基因转染。“流体力学”注射后所造成的肝窗扩大和肝细胞膜通透性的增加,可能是肝内基因有效转染的重要机制。目前“流体力学方法”在基因研究的各个领域都得到了广泛的应用,文章重点介绍了该方法在基因功能、疾病基因治疗和获得基因产物等几个方面研究中的应用。同时也简要介绍了增强外源基因在靶器官内表达的进展。

As a simple, convenient and efficient in vivo transgene method, hydrodynamics-based transfection provides biogeneticists with an important tool for gene study. High levels of foreign gene expression in liver can be achieved by rapid tail vein injection of a large volume of a naked DNA ment mech procedure has been used in many fields of gene studies, including gene function study, disease treatment, gene product obtaining etc. The article also covers the methods for the in vivo enhancement of exogenous gene expression in target organs.