摘要
目的建立心可舒片中葛根素的含量测定方法。方法RP-HPLC法。色谱柱：Zorbax Eclipse XDB- C_(18)(4．6mm×150mm,5μm)；流动相：甲醇-水-磷酸(23∶77∶0．5)；检测波长：250nm；流速：1．0mL·min^(-1)；柱温：25℃。结果葛根素在27．1～271．0 ng范围内线性关系良好,回归方程Y=4．664×10~3X(r=0．999 8),平均加样回收率为100．5%,RSD为1．8%(n=5)。结论本方法操作简便、准确快速、重现性好,可用于心可舒片的质量控制。
Objective To establish a RP - HPLC method for determining the content of puerarin in Xinkeshu Tablets. Method The analysis was carried on a column of Zorbax Eclipse XDB - C18（4.6 mm × 150 mm, 5 μm） at 25 ℃ , with methanol - water - phosphoric acid （23： 77： 0. 5） as mobile phase. The flow rate was 1.0 mL · min^-1, and the detection wavelength was 250 nm. Result A good linearity was found in the range of 27.1 - 271.0 ng. The average recovery rate was 100. 5 %, and RSD was 1.8% （n = 5）. Conclusion The method is simple, rapid, accurate, reproducible, and can be used to control the quality of Xinkeshu Tablets.
出处
《中药新药与临床药理》
CAS
CSCD
2006年第6期455-457,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
