摘要
目的:建立益肾十七味丸中乌头碱的含量测定方法。方法:采用高效液相色谱法测定乌头碱的含量,色谱柱为ODS柱;流动相为甲醇-0.1%三乙胺(80:20);检测波长230nm。结果:乌头碱在0.14~0.841μg范围内呈良好的线性关系,加样平均回收率为98、98%,RSD为1.60%。结论:本法简单,快速,结果准确,可作为该制刑的质量控制方法。
Objective: To establish a method for assay of aconitine in Yishen - shiqiwei Pill. Methods: HPLC was carried out on ODS column with methanol - 0.1% trielhylamine(80:20) as mobile phase, the detection wavelength was 230nm.. Results:The linear range of aconitine was 0.14 ~ 0.84μg, the average recovery was 98.98 %, RSD was 1.60%. Conclusion: This method is simple, rapid, accurate and available for quality control of the preparation.
出处
《中国民族医药杂志》
2006年第6期69-70,共2页
Journal of Medicine and Pharmacy of Chinese Minorities