坐骨神经损伤对局部淋巴结形态学影响的研究

Effects of the sciatic nerve defect on the regional lymph node morphology

目的:通过研究坐骨神经损伤对局部淋巴结组织形态学的影响,探讨周围神经损伤对局部免疫反应的作用。方法:建立小鼠右侧坐骨神经损伤模型,在术后1、2、4、8周观察坐骨神经损伤后下肢腘窝淋巴结组织的形态学变化;荧光显微镜检测局部淋巴结组织IgG表达变化;透射电子显微镜观察其超微结构变化。结果:神经损伤后各组局部淋巴结组织中淋巴窦内出现较多的浆细胞;免疫组化结果表明局部淋巴结IgG阳性细胞明显增加,其数量在第2、4周与正常组和假手术组比较差异有统计学意义(P<0.05);神经损伤后各组局部淋巴结的超微结构中出现较多巨噬细胞与树突状细胞,可见浆细胞成堆出现,胞浆内粗面内质网增生并呈囊样扩张,以术后1周最明显。结论:周围神经损伤后可以引发局部淋巴器官的形态学改变。

Objective ：To study the effect of the sciatic nerve defect on the regional lymph node morphology and to explore the effect of peripheral nerve injury on local immune reaction. Methods ：The mouse model of right sciatic nerve transection was made.The changes in the morphology of the regional lymph node by light microscope.The ultrastructure of the regional lymph node by transmission electron microscope.The IgG positive cells in the regional lymph node by immunofluorescence histochemistry. Results ： Much more plasma cells were found in lymph sinuses and in regional lymph nodes after sciatic nerve tramsusection.Immunohistochemistry showed that IgG positive cells increased remarkably in the experiment group, and there was statistical significance at the second and the fourth week compared with the normal group and the sham operation group （P〈0.05）. A great many of macrophages and dendritic cells were found and plasma cells piled up with rough endoplasmic reticulum distention in the ultramicrostructure of regional lymph nodes in experiment group,especially at the first week after operation. Conclusion：Sciatic nerve transection may result in morphology changes of regional immune organ.