摘要
以金桂、银桂、丹桂、四季桂四种桂花的茎尖为试验材料,采用均匀设计U6(63)方案,分析了基本培养基(B5、LMC)附加不同浓度的6-BA及NAA对四种桂花的茎尖丛生芽诱导的影响,研究结果表明:①适合这几种桂花丛生芽分化的最佳培养基金桂为:B5+6-BA 2．0+NAA 0．12。银桂为:B5+6-BA7．0+NAA0．12,丹桂为:B5 +6-BA7．0,四季桂仅分化出少量的丛生芽;②品种之间的增殖率差异表现为:金桂>银桂>丹桂>四季桂。
Study was carded out on effect of basic medium （ B5,LMC ） with different hormones （ 6-BA, NAA ） on induction of bunch buds from stem apex culture of four Osmanthusfragrans cultivars （ O. fragrans ＇Jingui＇, O. fragrans ＇Dangui＇, O. fragrans ＇Yingui＇ and O. fragrans ＇Sijigui＇ by applying uniform design U6 （ 6^3 ） scheme. The result showed that the optimal medium for them were as follows： B5 ＋ 6-BA2.0 ＋ NAA0.12 for O. fragrans ＇Jingui＇; B5 ＋ 6-BA7.0 ＋ NAA0.12 for O. fragrans＇Yingui＇; B5 ＋ 6-BA7.0 for O. fragrans＇Dangui＇. O. fragrans＇Sijigui＇ induced only few buds. The differentiation ratio among the cultivars is： O. fragrans＇Jingui＇〉 O. fragrans ＇Yingui＇〉 O. fragrans ＇Dangui＇〉 O. fragrans ＇Sijigui＇.
出处
《浙江林业科技》
北大核心
2006年第5期24-27,共4页
Journal of Zhejiang Forestry Science and Technology
关键词
均匀设计
金桂
银桂
丹桂
茎尖培养
uniform design
Osmanthusfragrans
stem apex culture
