摘要
以金桂、银桂、丹桂、四季桂四种桂花的茎尖为试验材料,采用均匀设计U6(63)方案,分析了基本培养基(B5、LMC)附加不同浓度的6-BA及NAA对四种桂花的茎尖丛生芽诱导的影响,研究结果表明:①适合这几种桂花丛生芽分化的最佳培养基金桂为:B5+6-BA 2.0+NAA 0.12。银桂为:B5+6-BA7.0+NAA0.12,丹桂为:B5 +6-BA7.0,四季桂仅分化出少量的丛生芽;②品种之间的增殖率差异表现为:金桂>银桂>丹桂>四季桂。
Study was carded out on effect of basic medium ( B5,LMC ) with different hormones ( 6-BA, NAA ) on induction of bunch buds from stem apex culture of four Osmanthusfragrans cultivars ( O. fragrans 'Jingui', O. fragrans 'Dangui', O. fragrans 'Yingui' and O. fragrans 'Sijigui' by applying uniform design U6 ( 6^3 ) scheme. The result showed that the optimal medium for them were as follows: B5 + 6-BA2.0 + NAA0.12 for O. fragrans 'Jingui'; B5 + 6-BA7.0 + NAA0.12 for O. fragrans'Yingui'; B5 + 6-BA7.0 for O. fragrans'Dangui'. O. fragrans'Sijigui' induced only few buds. The differentiation ratio among the cultivars is: O. fragrans'Jingui'〉 O. fragrans 'Yingui'〉 O. fragrans 'Dangui'〉 O. fragrans 'Sijigui'.
出处
《浙江林业科技》
北大核心
2006年第5期24-27,共4页
Journal of Zhejiang Forestry Science and Technology
关键词
均匀设计
金桂
银桂
丹桂
茎尖培养
uniform design
Osmanthusfragrans
stem apex culture