摘要
以夏蜡梅茎段为外植体,进行夏蜡梅组培试验,结果表明:外植体茎段的最佳消毒方式是先用70％酒精消毒10-15 s,再用0．1％HgCl溶液浸泡3-5 min;继代增殖培养的最佳培养基为MS+6-BA2.0mg/L+ NAA 0．1mg/L+蔗糖30 g/L,增殖系数4．03,茎芽生长粗壮;最佳生根培养基为1/2MS+IBA0．4mg/L。
Experiment on tissue culture of Calycanthus chinensis by using stems as explant showed that the optimal sterilizing method was dipping the stems in 70% alcohol for 10 - 15 seconds and 0.1% Hgcl for 3 - 4 minutes. The optimum medium for multipfication was MS ＋ 6-BA2.0mg/L ＋ NAA0.1mg/L ＋ sugar 30 g/L, and the rate of regeneration reached 4.03 with the IBA concentration of 0.4 mg/L in 1/2MS.
出处
《浙江林业科技》
北大核心
2006年第5期28-30,共3页
Journal of Zhejiang Forestry Science and Technology
关键词
夏蜡梅
组织培养
增殖
生根
Calycanthus chinensis
tissue culture
multiplication
rooting
