β-雌二醇聚氰基丙烯酸正丁酯纳米粒制备工艺的研究

Study on the preparing technology of β-estradiol polybutylcyanoacrylate nanoparticles

目的制备β-雌二醇聚氰基丙烯酸正丁酯纳米粒(E2-PBCA-NP),考察影响纳米粒粒径大小、包封率的因素,并优化其制备条件。方法以氰基丙烯酸正丁酯为载体,采用界面聚合的方法制备E2-PB-CA-NP,以粒径大小、形态和包封率为指标,通过单因素试验初选,正交设计法优化E2-PBCA-NP制备工艺。结果搅拌速度、浓缩温度、有机溶剂种类及表面活性剂类型均可影响粒径。按优化工艺条件,制得的载药纳米粒平均粒径为115nm,分布范围50～180nm,包封率90.3%。结论经过优化筛选出的工艺,为制备E2-PB-CA-NP的最佳工艺。

[Objective] To prepare beta-estradiol polybutylcyanoacrylate nanoparficles （E2-PBCA-NP）, study the factors affecting the particle size and the envelope rate, and optimize the preparing technology. [Method] E2- PBCA-NP was prepared by interracial polymerization with the butyleyanoaerylate as its carrier, the preparing technology was optimized by single factor test and orthogonal design test with particle size and the envelope rate as the test standard. [ Result] Various parameters including stirrlng rate, reaction temperature, organic solvent and the kind of surfaetas, could affect the particle size. Under the optimum condition, the average diameter of the nanoparticle was 115 nm, the distributing range between 50 and 180 nm, the envelope rate（90.3%）. [Conclusion] The optimized technology is the best one in preparing E2-PBCA-NP.