猪繁殖与呼吸障碍综合征病毒RT-PCR检测方法的建立

Establishment of Diagnose Method of RT-PCR to Porcine Reproductive and Respiratory Syndrome

设计了一对特异性引物,对16头临床症状、病理变化和ELISA检测呈阳性的猪繁殖与呼吸障碍综合征疑似病例的肺脏、脾脏和淋巴结以及种公猪的精液进行了RT-PCR扩增。结果显示16头样品的相应组织及精液都扩增出特异性基因片段,阳性符合率为100%,8头猪繁殖与呼吸障碍综合征血清ELISA检测为阴性的对照样品中有2头扩增出阳性片段,检出率为25%。RT-PCR检测方法的建立为猪繁殖与呼吸障碍综合征的准确诊断提供了技术手段。

A pair of peculiar primer was designed.16 suspectable cases of porcine reproductive and respiratory syndrome(PRRS) were tested whose clinical manifestation,pathological changes and ELISA were positive.The swine lungs,spleen and lymph node as well as boar seminal fluid were amplified using reverse transcription-polymerase chain reaction(RT-PCR).The result showed that the 16 samples are all amplified target gene fragments.The positive conformable probability is 100%.The amplifiation of two samples is positive in the comparative samples of the negative by ELISA to 8 suspect cases of PRRS serum.The detection rate is 25%.The establishment of RT-PCR provides a new method for the accurate detection of PRRS.