Identification of three novel noncoding RNAs from Drosophila melanogaster

三新奇小非编码的 RNA 从 D 的保存 intronicregions 被识别。由使用比较基因组学方法和分子的生物学途径的 melanogaster。Oneis 以一个 combinated 方法的新奇 snoRNA，它 snoRNAfamily 显示典型地代表 C/D 盒子的结构的特征并且为在 C2673 指导 D.melanogaster 28S rRNA 的 2''-O-methylation 拥有一个 10-nt-long rRNA 反感觉元素。另外的二是预言先锋采用已知的 miRNA 的 astem 环结构特征的 miRNAs。二 miRNAs 基因看起来有无所不在的表示侧面， ~ 23-nt RNA 抄本由北弄污检测了。我们的学习 revealed396 多种类 intronic 保存了与长度 from100 在 introns 嵌套到 500 bp 的序列(MCIS ) 。除了编码的小 RNA， MCIS 可能作为涉及基因抄写或 post-transcriptional 处理的 c/s-acting 元素工作。

Three novel small noncoding RNAs were identified from the conserved intronic regions of D. melanogaster by using comparative genomics method and molecular biology approach. One is a novel snoRNA in a combinated way, which displays structural features typical of C/D box snoRNA family and possesses a 10-nt-long rRNA antisense element for guiding the 2＇.-O-methylation of the D. melanogaster 28S rRNA at C2673. The other two are miRNAs whose predicted precursors adopt a stemloop structure characteristic of known miRNA. The two miRNAs genes appear to have ubiquitous ex- pression profiles with -23-nt RNA transcripts detected by Northern blotting. Our study revealed 396 multi-species intronic conserved sequences （MCIS） that nested in the introns with a length from 100 to 500 bp. In addition to small RNA coding, the MCIS might function as cis-acting elements involved in gene transcription or post-transcriptional processing.