组合抗体检测法诊断急性呼吸道感染

Diagnostic Value of Pathogenic Detection on Acute Respiratory Infection in Children by Multi-IgM Investigation

目的了解住院急性呼吸道感染患儿病原情况。方法对住院诊断急性呼吸道感染159例患儿，留取其血清标本，采用间接免疫荧光法，一次性同步进行呼吸道合胞病毒，腺病毒，流感病毒A型、B型，副流感病毒（1～4型），柯萨奇病毒B1型、A7型，埃可病毒7型和流感嗜血杆菌，肺炎克雷伯杆菌，百日咳杆菌，副百日咳杆菌及嗜肺军团菌血清1型、12型共18种病原血清IgM抗体检测。结果159例中18种常见呼吸道病原总阳性例数为103例（64．78％），检出频率由高到低依次为流感病毒A型66例（64．08％）、流感病毒B型49例（47.57％）、肠道病毒26例（25．24％）、呼吸道合胞病毒18例（17．48％）、副流感病毒11例（10.68％）；混合感染66例（64．08％），其中1／3为流感病毒A型和B型混合感染。结论病毒依然是儿童呼吸道感染的主要病原，其中流感病毒居首位。

Objective To investigate the pathogens of acute respiratory infection of children. Methods A total of 159 children with acute respiratory infection who were hospitalized in our department from August 2005 to January 2006 were involved in this study. The serum IgM antibody of 18 pathogens were detected by indirect immunofluorescence test. The 18 pathogens included respiratory syncytial virus （RSV）, adenovirus （ADV）, influenza A （H1N1, H3N2） and B viruses, parainfluenza viruses （PIV） type 1, 2, 3 and 4, coxsackie virus BI （CBV1）, coxsackie virus A7 （CAV7）, echovirus （ECHO7）, haemophilus influenzae （HI）, klebsiella pneumoniae （KP）, bordetella pertussis （BP）, bordetella parapertussis （BPP） and legionella pneumophila serotype 1 and 12. Results The evidence of specific IgM was obtained in 103 of 159 patients （64.78 %）. Influenza, a A was found in 66 cases （64.08 %）, influenza B in 49 cases （47.57 %）, enterovirus in 26 cases （25.24 %）, RSV in 18 cases （17.48 %）, HV in 11 cases （10.68 %）, and coinfection in 66 cases （64.08 %）, 1/3 of them were co - infected with influenza A and B. Conclusions Viruses are the most common agents of acute respiratory infection. Influenza virus is predominant among them.