肝素阻断呼吸道合胞病毒与呼吸道上皮细胞黏附作用

Value of Heparin Blocking Attachment of Respiratory Syncytial Virus to Human Airway Epithelial Cells

目的探讨呼吸道合胞病毒(RSV)与呼吸道上皮细胞的黏附机制。方法采用流式细胞仪定量检测RSV A2原型株与来自囊性纤维化患者的呼吸道上皮细胞株(CFBE)间的黏附效率,观察肝素及多种RSV抗体对黏附效应的影响。结果RSV与CFBE的黏附效率不及人喉淋状细胞癌细胞株(HEp-2)细胞,多种RSV抗体与RSV预孵后未能阻断其与CFBE黏附,但肝素却以剂量-效应依赖模式有效阻断黏附反应。结论CFBE细胞表面糖胺聚糖分子可能介导RSV与呼吸道上皮的黏附,采用流式细胞仪可直接定量评估RSV与靶细胞的黏附效率,优于病毒在靶细胞内繁殖的检测手段。

Objective To investigate the mechanisms of attachment of respiratory syneytial virus （RSV） to human airway epithelial cells. Methods Attachment of RSV to CFBE was quantitatively assessed by flow cytometry. Various polyclonal and monoelonal antibodies to RSV and heparin were pre - incubated with RSV. The blocking effects of these antibodies and heparin on attachment were evaluated. Results CFBE cells reduced capacity being hotrod by RSV. All the antibodies used were failed to block attachment of RSV on CFBE, whereas heparin blocked RSV attachment in a dose - response manner and the blockade by heparin was almost complete at the concentration of 0.4 mg/L. Conclusions Flow cytometry provides direct evaluation of attachment without growth of virus. Heparin - like molecules on cell surface of CFBE appears to be involved in attachment between RSV and human epithelial cells.