肺炎支原体肺炎患儿可溶性细胞间黏附分子-1的变化

Changes of Intercellular Adhesion Molecule-1 in Pathogenesis of Children with Mycoplasm Pneumoniae Pneumonia

目的检测肺炎支原体肺炎(MPP)患儿血清和诱导痰中可溶性细胞间黏附分子-1(SICAM-1)水平,探讨其在MPP发病机制中的作用。方法采用酶联免疫吸附试验(ELISA)检测住院的44例急性期、36例恢复期MPP和38例健康对照组儿童血清SICAM-1水平和诱导痰(年龄大能合作者:急性期34例、恢复期28例和对照组30例)中SICAM-1水平,白细胞总数(TC)、中性粒细胞(N)、淋巴细胞(L)、嗜酸性粒细胞(EO)、肥大细胞(Ma)以及巨噬细胞(Mφ)分类。结果MPP急性期患儿血清SICAM-1和痰SICAM-1水平、N、L、EO、Ma分类均明显高于恢复期和对照组(P均<0.05),Mφ明显低于恢复期和对照组(P均<0.05);3组患儿Tc比较无差异(P均>0.05);恢复期指标与对照组相比均无显著差异(P均>0.05)。急性期和恢复期并哮喘患儿血清和痰SICAM-1水平明显高于未并哮喘者(P<0.05)。急性期血清SICAM-1变化与痰SICAM-1、N、L、EO、Ma变化呈明显正相关(r=0.821,0.351,0.418,0.623,0.409 P均<0.05),与Mφ变化呈显著负相关(r=-0.394 P<0.05),与TC无相关性(r=0.287 P=0.07)。结论SICAM-1水平在MPP急性期患儿血清和痰中明显升高,并发哮喘患儿中尤为明显。实用儿科临床杂志,2006,21(22)

Objective To determine the levels of soluble intercellular adhesion molecule - 1 （SICAM - 1） in serum and induced sputum in children with mycoplasma pneumoniae pneumonia （MPP）, end to investigate the role of SICAM - 1 in pathogenesis of MPP. Methods The levels of SICAM - 1 in serum and induced sputum end sputum cell count were measured by enzyme linked immunosorbent assay （ELISA） kit in 44/34 children with MPP in acute episode period, 36/28 children in remission episode period and 38/30 healthy children. Results The levels of serum SICAM - 1, sputum SICAM - 1 end percent of neutrophil （N）, lymphocyte （L）, eosinophile （EO）, mast cell （Ma） were significantly higher in acute episode period in MPP than those in remission episode period and control group, respectively （all P 〈 0.05）, while percent of macrophage （MФ） decreased significantly （P 〈 0.05）. Total counts （TC） of cells in three groups showed no significant difference （P 〉 0.05）; the indexs in remission period and in controls showed no marked difference. The levels of serum end sputum SICAM - 1 in children with asthma attack in acute and remission episode periods in MPP increased significantly compared with those in children without asthma in both periods （P 〈 0.05）. There were positive correlation between serum SICAM - 1 and sputum SICAM - 1, N, L, EO, Ma, respectively （r = 0.821, 0.351, 0.418, 0.623, 0.409 all P 〈 0.05） and negative correlation between serum SICAM - 1 end MФ （r = -0.394 P 〈 0.05）. Conclusions The levels of serum end sputum SICAM - 1 increase significantly in acute period in MPP children, especially in MPP complicated asthma - attacked children.