高效液相色谱法测定强化面粉中V_A

Determination of Vitamin A in Fortified Flour by HPLC

建立了高效液相色谱（HPLC）测定强化面粉中维生素A含量的方法。样品用水-甲醇（5＋10）提取，提取液用正己烷萃取，萃取液直接用于色谱测定。实验以Chrosorb-60硅胶柱作为分离柱，经对色谱分离条件优化，最佳色谱分离条件为流动相为正己烷-异丙醇-乙酸比例为194：5：1（V／V／V）；流速为1．0mL／min；检测波长为326nm。实验结果表明，方法三水平样品回收率（n=5）为98．18％～100．72％；八次重复性实验相对标准偏差（RSD）为1．26％；检出限（S／N=3）为5ng。

A method of determination of vitamin A in fortified flour by （HPLC）has been developed. After the samples were extracted using water-methanol, the extract was filtrated . Vitamin A in the filtrate was extracted in hexane for HPLC measurement,on the Si column with the mobile phase composed of hexane： propanol-[2]： acetic acid solution （194：5： 1, V/V/V）. The flow rate of mobile phase was set at 1.0ml/min. The sample was detected by the UV detector at 326nm wavelength. The average recoveries were 98.18% - 100.72%. The relative standard devitations（RSD） was 1.26% （n = 8）. The detection limit （S/N = 3） was 5.0ng.