不同抗真菌药治疗白念珠菌性阴道炎模型小鼠的疗效比较

Comprasion of the Therapeutic Effectiveness of Three Different Anti-fungal Drugs in the Treatment of Vaginitis Caused by Candida Albicans in Mice

目的比较特比萘芬、氟康唑、伊曲康唑等抗真菌药治疗小鼠白念珠菌性阴道炎的疗效。方法构建ICR小鼠白念珠菌性阴道炎模型,应用特比萘芬、氟康唑、伊曲康唑灌服治疗,并在灌药后不同时间动态观察阴道灌洗液真菌载量。结果特比萘芬组在不同时间点其小鼠阴道灌洗液真菌载量明显高于氟康唑组及伊曲康唑组(P<0.01),连续灌药1周后其真菌载量仍处于较高水平。氟康唑组与伊曲康唑组从灌服药物后第2天开始其阴道灌洗液真菌载量明显减少,灌服药物后第3天开始阴道灌洗液真菌载量即处于极低水平,表明两组疗效显著,但两者之间疗效差异无显著性(P>0.05)。结论特比萘芬治疗小鼠白念珠菌性阴道炎的疗效欠佳,而氟康唑与伊曲康唑治疗小鼠白念珠菌性阴道炎疗效显著。

Objective To compare the therapeutic effectiveness of three different anti-fungal drugs terbindine, fluconazole and itraconazole in the treatment of experimental vaginitis caused by Candida albicans in mice. Methods The fungal vaginitis model was set up in female ICR mice by intravaginal inoculation of suspension of Candida albieans after the animals had been pretreated with estradiol. Mice divided randomly into different groups were then treated with terbinafine, fluconazole and itraconazole, respectively, administered by gastrogavage. The loading of the fungus in the vaginal lavage fluids in mice of the different groups were meatured dynamically at different time points after the beginning of the drug treatment. Results The fungal loadings in the vaginal lavage fluids taken at different time points from mice treated with terbinafine were significantly higher than those taken at corresponding time points from mice treated with fluconazole or itraconazole（ P 〈 0.01 ）. The fungal loading in the vaginal lavage fluids taken from mice 1 week after the beginning of the treatment with terbinafine remained at a relatively high level, A dramatic fall of the fungal loading in the vaginal lavage fluids taken on the 2nd day of treatment from mice treated with itraconazole or fluconazole group was demonstrated and the fungal loadings on the 3rd day of treatment in these mice were shown to be at a very low level, showing that itraconazole and fluconazole were highly effective in the treatment. However, the difference between the therapeutic effectiveness of these 2 drugs was not significant （ P 〉 0.05 ）. Conclusion Itraconazole and fluconazole, but not terbinafine, were shown to be very effective in the treatment of fungal vaginitis caused by Candida albicans in mice.