海洋放线菌M313菌株的鉴定及其抗肿瘤活性代谢产物的初步研究

Preliminary study of cytotoxic metabolite produced by marine actinomycete M313

按经典的放线菌分类方法对海洋放线菌M313菌株进行鉴定,用MTT检测法对海洋放线菌M313代谢产物的细胞毒活性与培养基的关系、活性物质的理化性质进行了研究。结果表明,该菌株可初步鉴定为孢杆链霉菌的海洋变种(Streptomycessporovirgulisvar.marinus);该菌株的发酵液对7种肿瘤细胞均具有很强的生长抑制活性,ID50为800～2000;M313菌株在GPDA、高氏一号培养基中均能产生细胞毒性很强的代谢产物;活性物质在pH2～10稳定,但对热不稳定,60℃以上加热处理丧失大部分活性;活性成分可能为分子质量大于2000u的水溶性物质。

The marine actinomycete M313 was identified by the classical method of actinomycete taxonomy. The cytotoxicity of the metabolite produced by marine actinomyce M313 from different media was assayed by MTT method in Vitro, and physiochemical characteristics of the metabolite were evaluated. The results showed that the actinomycete M313 was primarily identified as Streptomyces sporovirgulis var. marinus. Its fermentation broth had strong cytotoxicity against seven varieties of tumor cells. Its ID50 ranged from 800 to 2 000. The GPDA and GS media were suitable for production of strong cytotoxic metabolites. The active substances are stable under the pH 2-11, but are not heat-resistant above 60℃. The bioactive fractions were water-soluble and their molecular weight may be over 2 000 u.