IL－1α诱导牛脑微血管内皮细胞释放血小板衍生性生长因子

Effects of interleukin-1α on platelet-derived growth factor release from bovine cerebral microvascular endothelial cells

目的：研究白细胞介素－１α（ＩＬ－１α）诱导牛脑微血管内皮细胞（ＢＣＭＥＣ）释放血小板衍生性生长因子（ＰＤＧＦ），以及药物对由之引起的牛脑微血管平滑肌细胞（ＢＣＭＳＭＣ）增殖的拮抗作用。方法：体外培养ＢＣＭＥＣ和ＢＣＭＳＭＣ，结晶紫染色法测定细胞增殖。结果：ＩＬ－１α不能直接促进ＢＣＭＳＭＣ的增殖；但经ＩＬ－１α刺激的ＢＣＭＥＣ培养上清能显著地促进ＢＣＭＳＭＣ的增殖。这种增殖作用与ＩＬ－１α剂量呈正相关，并且可被抗ＰＤＧＦ抗体中和。欧芹素乙（Ｉｍｐ），异欧芹素乙（ｉｓｏ－Ｉｍｐ），６－（α，α－ｐｈｅｎｙ－ｌａｃｅｔｙｌｐｉｐｅｒａｚｉｎｙｌ）ｐｈｅｎｙｌ－５－ｍｅｔｈｙｌ－４，５－ｄｉｈｙｄｒｏ－３（２Ｈ）－ｐｙｒｉｄａｚｉｎｏｎｅ（ＰＭＤＰ）不影响ＩＬ－１α诱导ＢＣＭＥＣ释放ＰＤＧＦ，但对ＰＤＧＦ促ＢＣＭＳＭＣ增殖呈剂量依赖的拮抗。结论：ＩＬ－１α促进ＢＣＭＥＣ释放ＰＤＧＦ。ＩＬ－１α对ＢＣＭＳＭＣ增殖的促进作用需经ＰＤＧＦ等生长因子的介导。Ｉｍｐ，ｉｓｏ－Ｉｍｐ，ＰＭＤＰ拮抗ＰＤＧＦ引起的ＢＣＭＳＭＣ增殖。

Objective: To study the effect of interleukin-1α (IL-1α) on platelet-derived growth factor (PDGF)release from bovine cerebral microvascular endothelial cells (BCMEC),and the protection of drugs on proliferation of bovine cerebral microvascular smooth muscle cells (BCMSMC).Methods:BCMEC and BCMSMC were cultured in vitro and the proliferation of BCMSMC stimulated by PDGF was tested. Results : IL-1α did not affect the proliferation of BCMSMC directly. However, the conditioned medium of BCMEC stimulated by IL-1α promoted the proliferation of BCMSMC significantly in an IL-1αdose-dependent manner,and the mitogenic activity could be neutralized by anti-PDGF.Imperatorin(Imp),iso-Imperatorin (iso-Im p),6-(α,α-phenylacetylpiperazinyl) phenyl-5-met hyl-4, 5-dihydro- 3 (2H)-pyridaZinone (PMDP) did not affect the PDGF releasing from BCMEC stimulated by IL-1α, but inhibited the BCMSMC proliferation promoted by PDGF in a dose-dependent manner.Conclusion: IL-1α stimulates BCMEC to release PDGF.The proliferation of BCMSMC promoted by IL-1αmust be mediated by growth factors.such as PDGF.Imp,iso-Imp and PMDP inhibit proliferation of BCMSMC stimulated by PDGF.